Abstract:

OBJECTIVE: To investigate the acute toxicity and genetic damage in experimental animals. METHODS：In the acute toxicity study，mice were intragastrically administered by 21.5，10.0，4.64，2.15 g/kg rabdosia serra teabags groups and calculated the median lethal dose (LD50) by Horn method．In Ames test，set 5 000， 1 000，200，40，8 μg rabdosia serra teabags per plate．In mice bone marrow cell micronucleus and mice sperm abnormality test，2.50，5.00 and 10.0 g/kg rabdosia serra teabags groups．RESULTS：The acute oral toxicity in rabdosia serra teabags was more than 21.5 g/kg．In Ames test with and without S9 metabolic activation，the reverse mutations of typhimurium TA97， TA98，TA100，TA102 were less than double compared with natural mutations，and no dose-response relationship was found．The micronucleus rates and sperm abnormality rates in all doses showed no significant difference from the negative control (P>0.05). CONCLUSION：Under these experimental conditions，rabdosia serra teabags was shown to be a non-toxic substance. It did not cause genetic toxicity.

Key words: rabdosia serra, acute toxicity, ames test, micronucleus test, sperm abnormality test, genetic toxicity