用TK基因突变试验评价土荆芥精油的遗传毒性

doi:10.3969/j.issn.1004-616x.2014.05.013

Abstract

Abstract:

OBJECTIVE：The genotoxicity of essential oil from Chenopodium ambrosioides L. was evaluated with TK gene mutation test to provide information for its safety. METHODS：The mouse lymphoma cells L5178Y were exposed to essential oil of C. ambrosioides at volume fraction concentrations of 0.002%， 0.004%，0.006% and 0.008% for 3 h. 1% dimethyl sulfoxide (DMSO) and 10 μg/mL cyclophosphamide (CP) were used as solvent and positive controls，respectively. TK gene mutation was assessed with microwell method to detect cytotoxicity and mutation frequency at TK locus in L5178Y cells. RESULTS：Relative survival (RS)，relative suspension growth (RSG) and relative total growth (RTG) decreased when concentration of essential oil from C. ambrosioides increased，with only 29.38%，38.13% and 5.75%，respectively，in 0.008% dose group indicating significant cytotoxicity. Compared with the solvent control mutation frequency (TMF) increased with the rising concentration of essential oil(F=410.5，P=0.00). Multiple comparisons of TMT between the different essential oil groups，positive control group and solvent control group were statistically significant differences (P<0.05). The percentage of small growth mutant (SCM) was more than 95%. CONCLUSION： Essential oil from C. ambrosioides showed strong cytotoxicity and could increase mutation frequency at TK gene locus in L5178Y cells.

Key words: Chenopodium ambrosioides L, essential oil, mouse lymphoma cells L5178Y, TK gene mutation test, genotoxicity

WANG Ya-nan，LI Xin-xin，WANG Yu，ZHANG Du-yu，YANG Jun，MA Dan-wei. Evaluation of the genetic toxicity of essential oil from Chenopodium ambrosioides L. with TK mutation assay[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.05.013.

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Evaluation of the genetic toxicity of essential oil from Chenopodium ambrosioides L. with TK mutation assay

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