邻苯二甲酸二(2-乙基己)酯对大鼠卵巢病理和超微结构的影响

doi:10.3969/j.issn.1004-616x.2014.05.011

Abstract

Abstract:

OBJECTIVE: To explore the pathological damage and ultrastructure changes in female rat ovary when rats were treated with di(2-ethylhexyl) phthalate (DEHP)，to assess the toxicity of DEHP in female reproduction. METHODS：40 female SD rats were randomly divided into control group (corn oil)，DEHP low dose group (100 mg/kg)，DEHP medium dose group (500 mg/kg )，DEHP high dose group (1 500 mg/kg). The rats were treated with DEHP by gavage for 6 weeks. Rat body weight and ovary wet weight and ovary coefficients were calculated，the ovarian pathology was studied after HE staining，the ultrastructure changes in granulose cells were examined through electron microscopy. RESULTS：The ovarian weight and ovary coefficients were increased in DEHP high dose group. Pathological changes showed ovarian follicle damage，loose granular cell structure， follicular atresia after DEHP treatment. We also found mitochondria swollen， mitochondrial cristae decreased， cytoplasmic vacuolation， nuclear chromatin condensation，apoptotic bodies present，cell degeneration and necrosis in DEHP treated groups. CONCLUSION：DEHP could affect follicular development，induce granulose cell apoptosis in female rats，and subsequently causing reproductive toxicity.

Key words: di(2-ethylhexyl) phthalate, ovary, ovarian follicles, granulose cells, ultrastructure, reproductive toxicity

TAN Qin，QIN Xiao-yun，XU Xin-yun，ZHANG Qian，MA Quan，YANG Xi-fei，WU De-sheng，JIANG Ling-zhi，ZHOU Li，LIU Jian-jun，HUANG Hai-yan，YANG Lin-qing，HUANG Xin-feng，HUANG Guan-qin. Pathological damage and ultrastructure changes in female rat ovary induced by di(2-ethylhexyl) phthalate[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.05.011.

References

[1] 翟青枝，姚元庆. 邻苯二甲酸二(2-乙基己)酯对女性生殖系统的影响[J]. 中国妇产科临床杂志，2013，14(3)：286-288.

[2] Lovekamp-Swan T，Davis BJ. Mechanisms of phthalate ester toxicity in the female reproductive system[J]. Environ Health Perspect，2003，111(2)：139-145.

[3] 刘淑敏，常薇. 邻苯二甲酸酯致雌性生殖系统毒性及其机制的研究进展[J]. 环境与健康杂志，2013，30(4)：374-377.

[4] Schmidt JS，Schaedlich K，Fiandanese N，et al. Effects of di(2-ethylhexyl) phthalate (DEHP) on female fertility and adipogenesis in C3H/N mice[J]. Environ Health Perspect， 2012，120(8)：1123-1129.

[5] Takai R，Hayashi S，Kiyokawa J，et al. Collaborative work on evaluation of ovarian toxicity. 10) Two- or four-week repeated dose studies and fertility study of di-(2-ethylhexyl) phthalate (DEHP) in female rats[J]. J Toxicol Sci，2009，34 (Suppl 1)：111-119.

[6] 李娜. DEHP及其代谢产物MEHP对雌性大鼠生殖毒性及相关机制研究[D]. 吉林：吉林大学，2013.

[7] 李娜. DEHP对雌性小鼠生殖毒性的研究[D]. 吉林：吉林大学，2010.

[8] Grande SW，Andrade AJ，Talsness CE，et al. A dose-response study following in utero and lactational exposure to di-(2-ethylhexyl) phthalate (DEHP)：Reproductive effects on adult female offspring rats[J]. Toxicology，2007，229(1/2)：114-122.

[9] 王晓红. 邻苯二甲酸脂类化合物对大鼠卵巢功能的影响[D]. 广东：中山大学，2010.

[10] 刘鹏，李向臣，姚雅馨，等. 卵巢颗粒细胞凋亡的调控因素[J]. 现代生物医学进展，2009，9(13)：2562-2564.

[11] 沈强，俞彰，法京，等. 电镜下几种凋亡细胞的形态特征[J]. 复旦学报，2010，37(3)：322-325.

[12] 张育辉，贾林芝，王宏元. 中国大鲵闭锁卵泡的纤维和超微结构观察[J]. 动物学报，50(4)：615-621.

[13] 彭远英，彭正松，喻可芳. 凋亡细胞的超微结构变化[J]. 细胞生物学杂志，2003，25(5)：280-283.

[14] Yu Y，Li W，Han Z，et al. The effect of follicle-stimulating hormone on follicular development，granulose cell apoptosis and steroidogene-sis and its mediation by insulin-like growth factor-Ⅰin the goat ovary[J]. Theriogenology，2003，60(9)： 1691-1704.

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Pathological damage and ultrastructure changes in female rat ovary induced by di(2-ethylhexyl) phthalate

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