邻苯二甲酸二(2-乙基己)酯对雄性大鼠激素相关基因表达水平的影响

doi:10.3969/j.issn.1004-616x.2014.05.010

Abstract

Abstract:

OBJECTIVE: To explore the effects of di(2-ethylhexyl) phthalate (DEHP) on hormonal-related gene expression levels in male rats. METHODS：A total of 40 healthy 5-week-old male Sprague-Dawley rats were randomly divided into four groups with 10 rats in each group. The DEHP doses were 0 mg/kg in control group(corn oil)，100 mg/kg in low dose group，500 mg/kg in medium dose group and 1 500 mg/kg in high dose group. Rats were exposed to DEHP via gavage administration for six consecutive weeks，DEHP administration was once a day and five times a week. After exposure，rats were anesthetized by ether，then sacrificed for further study. The relative gene expression levels of steroidogenic acute regulatory protein (StAR)，3β-hydroxysteroid dehydrogenase(3β-HSD)，17β-hydroxysteroid dehydrogenase (17β-HSD)，estrogen receptors (ERα/β) and gonadotropin releasing hormone (GnRH) were analyzed by real time quantitative RT-PCR. RESULTS：Compared with control group，the relative gene expression levels of StAR and 3β-HSD were increased in medium DEHP dose and high DEHP dose groups (P<0.01 or P<0.05). The relative gene expression levels of 17β-HSD，ERα and ERβ were increased in high DEHP dose group (P<0.01 or P<0.05)，while the relative gene expression levels of GnRH was decreased in medium DEHP dose and high DEHP dose groups(P<0.01 or P<0.05). CONCLUSION：DEHP could cause endocrine disorder and interfere with the synthesis of male hormones in rats，ultimately leading to male reproductive dysfunction.

Key words: di(2-ethylhexyl) phthalate, steroidogenic acute regulatory protein, 3β-hydroxysteroid dehydrogenase, 17β-hydroxysteroid dehydrogenase, gonadotropin releasing hormone, estrogen receptor α, estrogen receptor β, gene expression

QIN Xiao-yun，TAN Qin，XU Xin-yun，WU De-sheng，HUANG Hai-yan. The effects of di(2-ethylhexyl ) phthalate on hormonal- related gene expression levels in male rats'[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.05.010.

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The effects of di(2-ethylhexyl ) phthalate on hormonal- related gene expression levels in male rats'

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