Bcl-2蛋白在肿瘤放化疗氧化损伤中的作用

doi:10.3969/j.issn.1004-616x.2014.05.009

Abstract

Abstract:

OBJECTIVE: To evaluate role of protein Bcl-2 in the oxidative injury induced by radiotherapy or chemotherapy. METHODS：We employed the rat models for radiotherapy and chemotherapy. For each experiment，the rats were divided into four groups：control group，model group，radiotherapy or chemotherapy group，and protection group. The rats for radiotherapy experiment received local radiation of 60Co γ with the accumulative dosage of 47 Gy. The rats for chemotherapy experiment received doxorubicin (2 mg/kg) by intraperitoneal injection every two days. The protection groups received multiple antioxidants (Antitoxin 60 mg/kg+Vit E 20 mg/kg+Tannin 2.5 mg/kg) by gavage every day. These treatments lasted 5 weeks for radiotherapy experiment and 8 weeks for chemotherapy experiment. At the end of the experiments，all of the rats were sacrificed and the specific tissues were fixed to cut sections. The expression of protein Bcl-2 was measured by immunohistochemistry and the positive stainings were quantified by optical density analysis. RESULTS：There was a certain positive expression of protein Bcl-2 in the normal rats. Radiotherapy or chemotherapy treatment significantly inhibited the protein Bcl-2 expression (P<0.05)，in which antioxidant supplementations showed obvious protection (P<0.05). CONCLUSION：Protein Bcl-2 was a key target of oxidative injury induced by radiotherapy and chemotherapy，as well as a potential target of antioxidant supplementation to reduce the side effects of radiotherapy and chemotherapy.

Key words: Bcl-2, radiotherapy, chemotherapy, antioxidant, target

QIN Xu-jun，HE Wei，HAI Chun-xu*. Role of protein Bcl-2 in the oxidative injury induced by radiotherapy or chemotherapy[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.05.009.

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Role of protein Bcl-2 in the oxidative injury induced by radiotherapy or chemotherapy

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