不同种类细胞株对UVB紫外线的耐受性初探

doi:10.3969/j.issn.1004-616x.2014.05.008

Abstract

Abstract:

OBJECTIVE: To investigate the tolerance of various kinds of cell lines to ultraviolet B radiation. METHODS：Cell viability and proliferation were detected by MTT，firefly luciferase activity and cloning efficiency assays after cells including epidermal cells (HaCaT)，melanocytes (A875)，pulmonary adenocarcinoma cells (A549 and H322) and hepatocarcinoma cells (HepG2) of human were irradiated with ultraviolet B at dose of 20 or 50 mJ/cm2. RESULTS：Cell viability including epidermal cells (HaCaT)，melanocytes (A875)，pulmonary adenocarcinoma cells (A549 and H322) and human hepatocarcinoma cells (HepG2)，decreased significantly 1 day after ultraviolet B irradiation at doses of 20 and 50 mJ/cm 2 (P<0.05). The viability of these cells were still lower than untreated control cells 3 days after irradiation. Finally，the proliferative ability of all the examined cells was suppressed after ultraviolet B irradiation at doses of 20 and 50 mJ/cm2(P<0.05)，as shown by cloning efficiency assays. Further firefly luciferase activity assay indicated that the viability of A549 cells were suppressed 5 and 10 days after exposure(P<0.05). CONCLUSION：Ultraviolet B irradiation affected the viability and proliferation of epidermal cell，lung adenocarcinma cell and hepatoma cell. Normal epidermal cell HaCaT possessed the more tolerance than A875，A549，H322 and HepG2 cells which is the source of tumors on ultraviolet B.

Key words: UVB ultraviolet, cell viability, proliferation, tolerance

GAO Ling，LI Feng-sheng，FENG Jiang-bin，LI Xiang-wen，LIU Jian-xiang，LIU Qing-jie. Investigation of the tolerance of various kinds of cell lines to ultraviolet B radiation[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.05.008.

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Investigation of the tolerance of various kinds of cell lines to ultraviolet B radiation

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