155例汉族男性人群5-羟色胺受体1B基因单核苷酸多态性的特征分析

doi:10.3969/j.issn.1004-616x.2014.05.005

Abstract

Abstract:

OBJECTIVE：To investigate the genetic characteristics of single nucleotide polymorphisms (SNPs) in serotonin receptor 1B gene (HTR1B) in Chinese Han males. METHODS：SNPs located in HTR1B were identified in 155 cases of target population through sequencing in this study. The genetic characteristics，linkage disequilibrium (LD) of each polymorphism locus，tag SNPs，haplotypes and their blocks were analyzed with HaploView software. RESULTS：Seven SNPs were obtained： rs17273700(-860A>G)，rs1228814(-700G>T)，rs11568817 (-262A >C) and rs130058(-161T>A) in the 5′-flanking region，rs6298(+129A>G)，rs6296(+861G>C) in the coding region and rs6297(+1 180T>C) in the 3′-flanking region. All the allele frequencies were in the Hardy-Weinberg equilibrium (P>0.05). There were high LD between the polymorphisms of -860A>G and -262A>C. +129A>G and +861G>C also showed high LD in this population. Eight major haplotypes (H1-H8) accounting for 94.2% were established，while the frequency of wild type haplotype (H1) was 46.0%. -860A>G，-700G>T，-161T >A，+129A>G，+1 180T>C were determined as tag SNPs. Two haplotype blocks were constructed and -860A >G，+129A>G were determined as their htSNPs. CONCLUSION：The appropriate tag SNPs and htSNPs in HTR1B are established in Chinese Han males for the first time. It lowered the number of SNP loci in further research，and provided the basis for explorations of their association with diseases.

Key words: HTR1B gene, single nucleotide polymorphisms, haplotype

ZENG Fan-song，LI Wen，XIAO Bin，XU Guo-yong，CHEN Hui-feng，LIN Han-sheng，CHEN Qing-song，HUANG Han-lin. Analysis of serotonin receptor 1B gene single nucleotide polymorphisms in 155 Chinese Han men[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.05.005.

References

[1] Razzaque Z，Pickard JD，Ma QP，et al. 5-HT1B-receptors and vascular reactivity in human isolated blood vessels： assessment of the potential cardiovascular selectivity of sumatriptan[J]. Br J Clin Pharmacol，2002，53(3)：266-274.

[2] Nilsson T，Longmore J，Shaw D，et al. Characterisation of 5-HT receptors in human coronary arteries by molecular and pharmacological techniques[J]. Eur J Pharmacol，1999，372 (1)：49-56.

[3] Cao，JX，Hu J，Ye XM，et al. Association between the 5-HTR1B gene polymorphisms and alcohol dependence in a Han Chinese population[J]. Brain Res，2011，28(1376)：1-9.

[4] Susol E，Mac Gregor AJ，Barrett JH，et al. A two-stage，genome-wide screen for susceptibility loci in primary Raynaud's phenomenon[J]. Arthritis Rheum，2000，43(7)： 1641-1646.

[5] Henriksen R，Dizeyi N，Abrahamsson PA. Expression of serotonin receptors 5-HT1A，5-HT1B，5-HT2B and 5-HT4 in ovary and in ovarian tumours[J]. Anticancer Res，2012， 32(4)：1361-1366.

[6] León-Ponte M，Ahern GP，O'Connell PJ. Serotonin provides an accessory signal to enhance T-cell activation by signaling through the 5-HT7 receptor[J]. Blood，2007，109(8)：3139-3146.

[7] Kidd M，Gustafsson BI，Drozdov I，et al. IL1beta- and LPS-induced serotonin secretion is increased in EC cells derived from Crohn’s disease[J]. Neurogastroenterol Motil，2009，21(4)： 439-450.

[8] Iwabayashi M，Taniyama Y，Sanada F，et al. Role of serotonin in angiogenesis：induction of angiogenesis by sarpogrelate via endothelial 5-HT1B/Akt/eNOS pathway in diabetic mice[J]. Atherosclerosis，2012，220(2)：337-342.

[9] 杨淋清，杨杏芬，林忠宁，等. 中国南方粤语方言地区汉族人群GSTM1、GSTT1基因多态性[J]. 癌变·畸变·突变，2006，18(3)：185-189.

[10] 陈慧峰，林忠宁，林育纯，等. 蛋白磷酸酶2A-Aα亚基基因5’侧翼区多态性在广东汉族人群中的分布[J]. 癌变·畸变·突变，2011，23(2)：93-97.

[11] 陈青松，李文，郎丽，等. 5-羟色胺受体1B基因多态性及其与振动性白指关联分析[J]. 中国职业医学，2013， 40(1)：1-5.

[12] Carlson CS，Eberle MA，Rieder MJ. Selecting a maximally informative set of single nucleotide polymorphisms for association analysis using linkage disequilibrium[J]. Am J Hum Genet，2004，74(1)：106-120.

[13] Wall JD，Pritchard JK. Haplotype blocks and linkage disequilibrium in the human genome[J]. Nat Rev Genet，2003， 4(8)：587-597.

[14] 陈慧峰，林忠宁，林育纯，等. 广东人群PP2A-Aα亚基基因5′-侧翼区单体型分析[J]. 中国预防医学杂志，2011， 12(3)：219-222.

[15] Langefeld CD，Fingerlin TE. Association methods in human genetics[J]. Methods Mol Biol，2007，404：431-460.

[16] Huang YY，Grailhe R，Arango V，et al. Relationship of psychopathology to the human serotonin1B genotype and receptor binding kinetics in postmortem brain tissue[J]. Neuropsychopharmacology，1999，21(2)：238-246.

[17] Smoller JW，Biederman J，Arbeitman L，et al. Association between the 5HT1B receptor gene(HTR1B) and the inattentive subtype of ADHD[J]. Biol Psychiatry，2006，59(5)：460-467.

[18] Wall JD，Pritchard JK. Haplotype blocks and linkage disequilibrium in the human genome[J]. Nat Rev Genet，2003， 4(8)：587-597.

Analysis of serotonin receptor 1B gene single nucleotide polymorphisms in 155 Chinese Han men

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 9

Recommended Articles

Metrics

Comments

[1]	WANG Lan, ZHANG Yongdong, GUO Hongyun, WANG Tao, WANG Haitao, GUO Huan, YANG Suisheng, LI Xiaoqin, SU Haixiang. Relationships between XRCC3 polymorphisms and breast cancer in northern Chinese women [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(5): 392-396,400.
[2]	YING Wei, WANG Xingyuan, JIANG Bo, ZHOU Wei. Association between polymorphisms in the miR-146a and susceptibility to non-small cell lung cancer [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(3): 222-226.
[3]	XIAO Sha, ZHAO Xiujuan, KUANG Shicheng, LIU Yunru, LONG Wenfang, YANG Jianjun, HUANG Hairong. A Meta-analysis of the relationship between XPD 751 gene polymorphism and lung cancer [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(4): 292-297.
[4]	ZHANG Xiao-ping1，JIAN Zi-ying1，CHEN Bao-an，XU Pei-pei，PENG Miao-xin，XIA Guo-hua，CHENG Lu，LU Zu-hong. Detection of SNPs of DCK and CDA genes in five leukemia cell lines with MALDI-TOF MS [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2013, 25(2): 115-119.
[5]	CHEN Bao-an，ZHANG Wen-jing，ZHANG Xiao-ping，BAI Zhi-bin，FENG Ji-feng，ZHONG Yue-jiao，DING Jia-hua ，GAO Chong， BAO Wen，PENG Miao-xin， CHENG Lu， LU Zu-hong. Detection of single nucleotide polymorphisms of DPYD and MTHFR genes in colon cancer cell lines [J]. , 2012, 24(2): 96-99.
[6]	JIN Yi-feng1, LI Tie-chen2, WANG Ying1,,LIU Dong-hua11,SUN Zhen-gui1,. Single nucleotide polymorphisms in ERCC1 and XPD genes and sensitivity to platinum_based chemotherapy in non_small_cell lung cancer [J]. , 2010, 22(5): 374-378.
[7]	FAN Zu-peng1;PENG Qun-xin2;GAO Ge1;WU Wen-ting1;QIAN Ji1;CHEN Hong-yan1. Association between single nucleotide polymorphisms in human POLE1 and susceptibility to lung cancer [J]. , 2010, 22(4): 265-270.
[8]	Yin Dong1;Chen Yan2;6;Zhang Hui-xia2;Wang Hong-jiang1;Aheli1;Deng Yan-chao3;6;Ma Yan-qing4;Julaiti5. Study on relationship between tagSNPs and haplotype of hCHK2 gene and esophageal cancer in Kazakh and Han nationality in Xinjiang [J]. , 2010, 22(4): 287-291.
[9]	CHEN Yan1; LIU Ji-wen1; ZHAO Jiang-xia1; CUI Jun2; LI Ling2; A Si-ya2; ZHU Yan3; TIAN Wei1. Polymorphisms of δ-Aminole- vulinic Acid Dehydratase; Vit D Receptor and Genetic Suscep- tibility of Lead Posioning in Han; Uygur and Kazak Children [J]. , 2009, 21(4): 280-285.