甲基苄基亚硝胺诱导大鼠食管癌癌前病变食管组织中β-catenin表达及其Ser675位点磷酸化水平的变化

doi:10.3969/j.issn.1004-616x.2014.05.003

Abstract

Abstract:

OBJECTIVE: To find the changes of β-catenin and phosphorylated β-catenin (p-β-catenin) Ser675 in esophageal precancerous lesions in Wistar rats induced by methyl benzyl nitrosamine (MBNA). METHODS： Wistar rats received MBNA injections at dose of 3.5 mg/kg 2 times per week. At 10 weeks，the pathological changes of esophageal mucosa were examined. β-catenin mRNA level was measured by quantitative real-time PCR and the expressions of β-catenin protein and p-β-catenin(Ser675) were evaluated by Western blot. The location of β-catenin was detected by immunohistochemistry. RESULTS：Compared with normal group，the level of β-catenin mRNA was significantly decreased，the levels of β-catenin and p-β-catenin (Ser675) protein were significantly increased in treated group. CONCLUSION：The elevated protein levels of β-catenin and p-β-catenin (Ser675) were related with esophageal precancerous lesions in Wistar rats induced by MBNA ，and could be considered as an indicator of esophageal carcinoma.

Key words: methyl benzyl nitrosamine, esophageal carcinoma, precancerous lesions, β-catenin, Ser675, phosphorylation

SHI Wen-rong，LIU Yan*. Effects of methyl benzyl nitrosamine on β-catenin and p-β-catenin(Ser675) in esophageal precancerous lesions in Wistar rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.05.003.

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Effects of methyl benzyl nitrosamine on β-catenin and p-β-catenin(Ser675) in esophageal precancerous lesions in Wistar rats

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