Abstract:

OBJECTIVE: To study Survivin shRNA interference on CDK4 mRNA and protein levels of esophageal cells ECA109. METHODS：Survivin shRNA interference technology was applied to liposomes transfection esophageal ECA109 cells，RT-PCR and Western blot methods was applied to  evaluate genes expression of Survivin and CDK4. Flow cytometry was used to detect the proliferation of cell lines index changes after ECA109 esophageal cell transfection. RESULTS：After Survivin shRNA transfection of esophageal ECA109 cell，Survivin mRNA and protein expressions were obviously down-regulated. CDK4 mRNA expression was also down-regulated. Cell ratio was increased in G2 phase but decrease in S phase，with the cell cycle blocked. CONCLUSION：Survivin was effective on adjusting CDK4 gene expression at mRNA level. The role of silencing Survivin down-regulated expression of CDK4 mRNA，suggesting  that Survivin could be a new gene regulatory factor in the tumor signal control network.

Key words: Survivin, esophageal cancer, short hairpin RNA, RNA interference, CDK4