Abstract:

OBJECTIVE: To test expressions of Survivin，NF-κB and STAT3 in 40 cervical carcinoma tissue and 30 normal cervical tissue samples. The correlation between Survivin，NF-κB and STAT3 expression level and the clinical factors and pathology classification were analyzed，and the relationship and mechanism of Survivin，NF-κB and STAT3 in cervical carcinoma development were studied. METHODS：Trizol one step method was used to extract total RNA from both cervical carcinoma and normal tissues，mRNA reverse transcriptase cDNA，the polymerase chain reaction (RT-PCR half quantitative) method was used to measure Survivin，NF-κB and STAT3 mRNA expressions in cervical carcinoma and normal tissues. Further analysis of correlation between Survivin，NF-κB and STAT3 mRNA expression and the clinical pathological factors were performed. RESULTS：Survivin，NF-κB and STAT3 mRNA expressions in carcinoma tissue were significantly higher than the normal tissue (P<0.05). Survivin，NF-κB and STAT3 mRNA expression inⅡb-Ⅲa stage was significantly higher than Ⅰ-Ⅱa stage (P<0.01). The expression of Survivin mRNA in patients with lymph node metastasis was higher than those with no lymph node metastasis (P<0.01) . Survivin expression was related to STAT3 expression (r=0.483，P<0.05) and to NF-κB expression (r=0.377，P<0.05). CONCLUSION： Survivin，NF-κB and STAT3 expressions were upregulated in cervical carcinoma. All of these were closely related to the development and progression of cervical carcinoma.

Key words: cervical carcinoma, Survivin, NF-κB, STAT3