血浆中apoAⅠ和mTOR蛋白在维吾尔族妇女宫颈病变进程中的动态变化及意义

doi:10.3969/j.issn.1004-616x.2014.03.006

Abstract

Abstract:

OBJECTIVE: To explore the relationships of the dynamic changes of plasma apoAⅠcontent and mTOR activity with cervical carcinogenesis development in Uyghur women. METHODS：We collected 82 plasma samples from Uyghur women with cervicitis (CV) (22 cases)，cervical intraepithelial neoplasia (CIN) Ⅱ-Ⅲ (20 cases)，early stage (Ⅰ-Ⅱ) (20 cases) and advanced stage (ⅡB-Ⅳ) (20 cases) cervical squamous cell carcinoma， quantitatively determined the content of plasma proteins apoAⅠand the activity of mTOR by enzyme linked immunosorbent assay (ELISA) and statistical analysis，in order to analyze the correlation with the progression of cervical lesion and evaluate the accuracy，specificity and sensitivity of candidate plasma protein markers for early warning of cervical cancer. RESULTS：With the development of cervicitis to CINⅡ-Ⅲ，early stage or advanced stage cervical cancer，the changes of two plasma proteins altered gradually，with obvious decrease in the content of apoAⅠ，and increase in the activity of mTOR，with statistical differences for all (P<0.05). Correlation analysis of cervical cancer-specific protein indicators found that mTOR and apoAⅠshowed a low negative correlation (r＜0.4，P＜0.05). The sensitivity，specificity，and accuracy of detection，for early warning of cervical cancer of the mTOR protein were 94.0%， 60.0% and 80.0%，respectively；and for apoAⅠwere 70.1%， 60.0% and 29.0%，respectively. apoAⅠ/mTOR combined detection of sensitivity，specificity， and accuracy were 82.1%，60.0% and 54.5%，respectively. CONCLUSION：The dynamic changes of apoAⅠcontent and mTOR activity were important indicators of the procession of cervical lesions，and expected to assist with the early diagnosis of cervical cancer.

Key words: Uyghur women, cervical carcinogenesis, apoAⅠ, mTOR, plasma biomarker

GUO Xia，JIN Hua，Mayila·KAMILIJIANG，LIU Kai-jiang，Axiangu·HASIMU,Halidan·REYIMU，WU Gui-zhen，Abulizi·ABUDULA. Correlation of the dynamic changes of plasma apoA l content and mTOR activity with cervical carcinogenesis development in Uyghur women[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.03.006.

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Correlation of the dynamic changes of plasma apoA l content and mTOR activity with cervical carcinogenesis development in Uyghur women

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