Toll样受体基因多态性与EBV感染相关胃癌的易感性

doi:10.3969/j.issn.1004-616x.2014.03.003

Abstract

Abstract:

OBJECTIVE: To explore the relationship of Toll-like receptors 2 (TLR2) and TLR4 Thr399Ile gene polymorphism and the susceptibility to EBV-associated gastric carcinoma (EBVaGC) and EBV-negative gastric carcinoma (EBVnGC). METHODS：TLR2 gene promoter region - 196 to - 174 del polymorphism was detected by using the allele-specific polymerase chain reaction in 52 EBVaGCs，157 EBVnGCs and 94 normal controls. TLR4 gene Thr399Ile genotype and allele distribution were evaluated by PCR and restriction fragment length polymorphism (PCR-RFLP) in 50 EBVaGCs，67 EBVnGCs and 71 normal controls. The relationship between gene polymorphism in TLR2 and TLR4 coding genes and the susceptibility to EBVaGC and EBVnGC was analysed. RESULTS：There was no significant difference (χ2=3.180，P=0.075) between gastric cancer group and control group in genotype distribution of TLR2(-196 to -174 del)，but the del allele frequency of gastric cancer group was obviously higher than that of control group (χ2=4.875，P=0.027)，The risk of developing gastric carcinoma in del allele carriers was significantly higher than that in controls(OR=1.491，95%CI= 1.045-2.126). TLR2 (-196 to -174 del) genotype and the frequency of del allele showed no significant difference in EBVaGC and EBVnGC group (χ2=0.05，P=0.867). TLR4 gene Thr399Ile locus mutation was not found in EBVaGC，EBVnGC or normal control groups. The genotype distribution and allele frequency also revealed no statistical differences . CONCLUSION：TLR2 -196 to - 174 del alleles could be risk factors for gastric carcinoma，but its effects were not different in EBVaGC and EBVnGC. There was no obvious correlation in the genotype distribution of TLR2(-196 to -174 del) and TLR4 gene Thr399Ile and EBVaGC genetic susceptibility.

Key words: Toll-like receptor, gastric carcinoma, EBV, gene polymorphism

ZHAO Zhen-zhen，SHI Yuan-yuan，LIU Song，WANG Xiao-feng，LUO Bing*. Toll-like receptors gene polymorphism and susceptibility to EBV- associated gastric carcinoma[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.03.003.

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Toll-like receptors gene polymorphism and susceptibility to EBV- associated gastric carcinoma

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