纳米二氧化钛对大鼠NK细胞活性和相关细胞因子的影响

doi:10.3969/j.issn.1004-616x.2014.03.001

Abstract

Abstract:

OBJECTIVE: To explore the effects of titanium dioxide (TiO2) nanoparticles on Natural Killer (NK) cell activity and related cytokines in rats. METHODS：Sprague Dawley rats were randomly divided into 0.5，4 and 32 mg/kg TiO2 nanoparticles exposure groups，control group (0.9% sodium chloride solution)，and TiO2 micro-sized particles exposure group (32 mg/kg). Rats were treated by intratracheal instillation of these substances in suspension twice a week，a total of 28 consecutive days. NK cell population in peripheral blood was measured by flow cytometry. NK cell killing activity in the spleen was analyzed by lactate dehydrogenase (LDH) release assay. Cytokines IL-2 and INF-γ were measured by liquid phase chips method. RESULTS：The NK cell activity increased with the rise of TiO2 exposure dose. Significant difference in NK cell activity in the spleen was found in the group exposed to 32 mg/kg TiO2 nanoparticles compared with the control group (P＜0.05). No significant difference was found between control group and micro-sized TiO2 group (P＞0.05). Compared with micro-sized TiO2 group，NK cell activity was found to be significantly increased in 32 mg/kg TiO2 nanoparticles exposure group (P＜0.05). No significant change of NK cell populations，IL-2 or INF-γ was observed compared with control group and micro-sized group (P＞0.05). CONCLUSION：TiO2 nanoparticles could trigger systemic immune responses，and enhance NK cell killing activity in the spleen in the studied dosage range. However，further research is needed to confirm the long-term changes in NK cell populations，NK cell killing activity and related cytokines，as well as the potential mechanisms.

Key words: TiO2 nanoparticles, natural killer cell, immune response, cell activity, cytokines

FU Yan-yun，ZHANG Yan-qiu，MA Shu-mei，YIN Li-hong，PU Yue-pu，LIANG Ge-yu*. Effects of titanium dioxide nanoparticles on natural killer cell activity and related cytokines in rat[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.03.001.

References

[1] Nel A，Xia T，M?dler L，et al. Toxic potential of marterials at the nanolevel[J]. Science，2006，311(5761)：622-627.

[2] 王文斌，李国珍，张凤梅，等. 纳米二氧化钛对大鼠脑皮层组织结构及其氧化应激水平的影响[J]. 环境与健康，2013，30(2)：113-116.

[3] Liu R，Zhang X，Pu Y，et al. Small-sized titanium dioxide nanoparticles mediate immune toxicity in rat pulmonary alveolar macrophages in vivo[J]. J Nanosci Nanotechnol，2010，10(8)： 5161-5169.

[4] 李艳芬，刘冉，梁戈玉，等. 纳米二氧化钛经气管染毒对大鼠肺巨噬细胞免疫功能的影响[J]. 环境与职业医学，2008，25(2)：156-158.

[5] 刘焕亮，杨丹凤，张华山，等. 3种典型纳米材料致大鼠免疫毒性的作用[J]. 解放军预防医学杂志，2010，28(3)：163-166.

[6] 刘焕亮，张华山，杨丹凤，等. 纳米材料对大鼠外周血T淋巴细胞亚群的影响[J]. 解放军预防医学杂志，2011，29(5)： 320-323.

[7] Chang X，Fu Y，Zhang Y，et al. Effects of Th1 and Th2 cells balance in pulmonary injury induced by nano titanium dioxide[J]. Environ Toxicol Pharmacol，2013，37(1)：275-283.

[8] Li N，Duan Y，Hong M，et al. Spleen injury and apoptotic pathway in mice caused by titanium dioxide nanoparticules[J]. Toxicol Lett，2010，195(2/3)：161-168.

[9] Sang X，Zheng L，Sun Q，et al. The chronic spleen injury of mice following long-term exposure to titanium dioxide nanoparticles[J]. J Biomed Mater Res A，2012，100(4)： 894-902.

[10] Chen J，Dong X，Zhao J，et al. In vivo acute toxicity of titanium dioxide nanoparticles to mice after intraperitioneal injection[J]. J Appl Toxicol，2009，29(4)：330-337.

[11] Wang J，Zhou G，Chen C，et al. Acute toxicity and biodistribution of different sized titanium dioxide particles in mice after oral administration[J]. Toxicol Lett，2007，168(2)： 176-185.

[12] van Ravenzwaay B，Landsiedel R，Fabian E，et al. Comparing fate and effects of three particles of different surface properties：nano-TiO2，pigmentary TiO2 and quartz[J]. Toxicol Lett，2009，186(3)：152-159.

[13] Asa G，Elsa L，Linda S，et al. Lung exposure of titanium dioxide nanoparticles inducesinnate immune activation and long-lasting lymphocyte response in the Dark Agouti rat[J]. J Immunotoxicol，2011，8(2)：111-121.

Effects of titanium dioxide nanoparticles on natural killer cell activity and related cytokines in rat

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