微囊藻毒素-LR对秀丽线虫精子形成的毒性作用

doi:10.3969/j.issn.1004-616x.2014.02.013

Abstract

Abstract:

OBJECTIVE: To study the toxicity of spermiogenesis caused by MC-LR exposure.? METHODS：L4 him-5 strain males were exposed to MC-LR at 0.1，1，10 and 100 μg/L for 48 h. The ability of spermatid activation was measured by testing its rates in vitro. Spermatid size was measured to assess its competitiveness. Expression levels of the related genes were evaluated by qRT-PCR. Male fertility was measured by brood sizes of him-5 males. RESULTS：After 48-hour exposure，the 100 μg/L group showed reduced spermatid size (P<0.01). Exposures to 10 and 100 μg/L reduced the rates of spermatid activation (P<0.01). Expression levels of spe-10 and spe-15 were significantly decreased after exposure. No significant decreases in brood sizes were observed. CONCLUSION：MC-LR may impair the process of spermiogenesis.

Key words: Caenorhabditis elegans, microcystins-LR, spermiogenesis, reproductive toxicity

YANG Fei-fei，ZHANG Min-hui，YIN Li-hong，LI Yun-hui. Toxicity of microcystins-LR on spermiogenesis in Caenorhabditis elegans[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.02.013.

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Toxicity of microcystins-LR on spermiogenesis in Caenorhabditis elegans

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