辐射诱导染色体畸变的快速FISH方法的建立

doi:10.3969/j.issn.1004-616x.2014.02.009

Carcinogenesis, Teratogenesis & Mutagenesis

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Establishment of a rapid FISH method to analyze chromosome aberrations induced by Irradiation

LI Shuang，LU Xue，ZHAO Hua，FENG Jiang-bin，LIU Qing-jie

China CDC Key Laboratory of Radiological Protection and Nuclear Emergency，National Institute for Radiological Protection，Chinese Centre for Disease Control and Prevention，Beijing 100088，China

Received:2013-05-07 Revised:2013-09-27 Online:2014-03-30 Published:2014-03-30
Contact: 刘青杰，E-mail：qjliu@nirp.cn
About author:李爽(1985- )，女，北京市人，硕士，实习研究员，研究方向：辐射生物效应。
Supported by:
国家自然科学基金资助项目(81172593)

Abstract

Abstract:

OBJECTIVE: To establish a rapid fluorescence in situ hybridization (FISH) method and to analyze the chromosome aberrations induced by irradiation. METHODS：Human centromeric alpha satellite DNA was amplified and directly labeled with Cy3-dUTP or Fluorescein-12-dUTP by degenerate oligonucleotide priming-PCR (DOP-PCR) to prepare two pan-centromeric probes. Chromosome aberrations in lymphocytes of healthy irradiated peripheral blood samples with 60Coγ-rays were analyzed with FISH. RESULTS：Two directly labeled pan-centromeric probes hybridized to the centromeres of all 46 chromosomes. The established rapid FISH method could easily detect chromosome aberrations after hybridizing 5 h to 12 h in 37 ℃ and simple washing. CONCLUSION： FISH method established in this study could be use to rapidly detect the dicentrics，centric rings，and translocations induced by irradiation.

Key words: pan-centromeric probe, FISH, chromosome aberrations, degenerate oligonucleotide priming-PCR

LI Shuang，LU Xue，ZHAO Hua，FENG Jiang-bin，LIU Qing-jie. Establishment of a rapid FISH method to analyze chromosome aberrations induced by Irradiation[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.02.009.

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Establishment of a rapid FISH method to analyze chromosome aberrations induced by Irradiation

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