Carcinogenesis, Teratogenesis & Mutagenesis

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Expression and the clinicopathological significance of SOX2 in gastric cancer

HUANG Zheng-jie,JIANG Long,YOU Jun,ZENG Yue-yue,WU Bing-lin,CHEN Bai-sheng,FENG Qing-zhao,LUO Qi   

  1. 1. Department of Surgical Oncology, the First Affiliated Hospital of Xiamen University and Xiamen Cancer Center, Xiamen 361003; 2. The First Clinical Medical College, Fujian Medical University, Fuzhou 350004, Fujian, China
  • Received:2013-07-08 Revised:2013-12-04 Online:2014-03-30 Published:2014-03-30
  • Contact: 罗 琪,E-mail:luoqixmzsh@126.com
  • About author:黄正接(1974- ),男,福建省漳州市人,硕士,副主任医师,副教授,研究方向:胃肠道肿瘤。E-mail:h74zj@126.com

Abstract:

OBJECTIVE: To investigate the expression of sex determining region Y-box 2(SOX2) in gastric cancer and explore the role of SOX2 in the development of gastric cancer. METHODS:Total RNA was extracted from 52 gastric cancer tissues and normal gastric mucosa tissues and reverse transcribed into cDNA. Real-time fluorescence quantitative RT-PCR was performed for evaluation of the expression of SOX2 mRNA in these tissues. RESULTS:SOX2 expression in well-differentiated and moderately differentiated gastric cancer tissues were higher than that of poorly differentiation(t=3.09 and t=2.87,P<0.05). Gastric cancer tissues showed higher SOX2 expression of invasion(T1+T2) (t=2.16,P=0.037),lymph node metastasis (t=2.54,P=0.017) and Ⅰ-Ⅱ clinical stages (t=2.59,P=0.014) compared to deeper invasion(T3+T4),lymph node metastasis and Ⅲ-Ⅳ clinical stages. There was no significant difference in SOX2 expression in the cancerous tissues of patients with different sex or age (P>0.05). CONCLUSION:Low SOX2 expression might be associated with the development,invasion and metastasis of gastric cancer and may potentially be used as marker for diagnosis and predicting the prognosis of gastric cancer.

Key words: stomach neoplasms, sex determining region Y-box 2, real-time fluorescent quantitative PCR