去甲斑蝥素对人肺腺癌A549细胞的抑制作用

doi:10.3969/j.issn.1004-616x.2014.01.005

Abstract

Abstract:

OBJECTIVE: To study the effects of norcantharidin (NCTD) on human lung cancer cells，and investigate the mechanisms. METHODS：The growth inhibition of A549 cells treated with 0-240 μmol/L NCTD for 0-72 hours was analyzed by MTT assay. The recovery growth and proliferation of A549 cells treated with 0-120 μmol/L NCTD for 24 h was evaluated by MTT assay. The morphological changes of A549 cells treated with 40，50 and 60 μmol/L NCTD for 0-72 h were examined under inverted microscope. The apoptosis and cell cycle changes of A549 cells treated with 40-60 μmol/L NCTD were detected by flow cytometry. RESULTS：NCTD inhibited the growth of A549 cells in 30-240 μmol/L(P<0.05). Cell growth inhibition could hardly be detected 24 h after 30-240 μmol/L NCTD was removed from culture medium. The vitality of A549 cells was fully recovered in 5 d after 30-60 μmol/L NCTD was removed from culture medium. A549 cells showed G2-M phase block but no obvious apoptosis compared with the control group when treated with 40，50 and 60 μmol/L NCTD for 0-72 h. CONCLUSION： NCTD (40-60 μmol/L) inhibited human lung cancer A549 cell growth mainly by cell G2-M phase block.

Key words: norcantharidin, lung cancer, A549 cell, cell cycle, apoptosis

CUI Baodi，WANG Min，SUN Zhenxiao. The inhibition of norcantharidin on human lung adenocarcinoma A549 cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.01.005.

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The inhibition of norcantharidin on human lung adenocarcinoma A549 cells

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