微囊藻毒素LR对大鼠卵巢颗粒细胞氧化损伤和凋亡的影响

doi:10.3969/j.issn.1004-616x.2014.01.004

Abstract

Abstract:

OBJECTIVE: To study the effects and mechanism of microcystin LR(MC-LR)on oxidative damage and apoptosis in rat ovarian granulosa cells. METHODS：After 48 h exposure to MC-LR at 0，10，20 and 30 μg/mL，the level of intracelluar reactive oxygen species，superoxide dismutase (T-SOD) activity and MDA in culture medium，apoptosis and the mRNA changes of Bax and Bcl-2 expressions were evaluated. RESULTS：After MC-LR exposure at the dosage of 10，20 and 30 μg/mL for 48 h，the T-SOD activity was significantly suppressed (P<0.05). The concentrations of ROS and MDA in the 20 μg/mL and 30μg/ml groups were significantly reduced (P<0.05) when compared with control group. The correlation coefficient between dosage of MC-LR and ROS was 0.925(P<0.01)，between dosage of MC-LR and MDA was 0.811(P<0.01)，between dosage of MC-LR and T-SOD was -0.865(P<0.05). Cell assay indicated that the expression of Bax was up-regulated(P<0.05) and Bcl-2 down- regulated (P<0.05). The correlation coefficient between dosage of MC-LR and Bax was 0.972(P<0.01)，between dosage of MC-LR and Bcl-2 was -0.972 (P<0.01). CONCLUSION：MC-LR was capable of inducing oxidative damage of rat granulosa cells in vitro. MC-LR could cause apoptosis and changes in the expresons of Bax and Bcl-2 .

Key words: microcystin LR, ovaries granulosa cells, oxidative damage, apoptosis, Bax；Bcl-2

YANG Tao，CHEN Yu-ping，WANG Jing，REN Nan，CHEN Hua. Effects of microcystins on the oxidative damage and apoptosis of ovarian granulosa cells in rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.01.004.

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Effects of microcystins on the oxidative damage and apoptosis of ovarian granulosa cells in rats

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