静磁场对体外三维培养小鼠腔前卵泡发育成熟的影响

doi:10.3969/j.issn.1004-616x.2013.06.005

Carcinogenesis, Teratogenesis & Mutagenesis

Effects of static magnetic field on proliferation of mouse preantral follicles 3D cultured in vitro

LIN Gui-miao¹，MEI Shu-jiang²，*，LIN Su-xia¹，XU Gai-xia³，LI Hui¹，ZHU Yue-quan¹，LIN Xiao-tan¹，CAI Zhi-ming⁴，WANG Xiao-mei¹，*，MA Han-wu²，LI Yuan²，LU Yan²，QIN Yan-min²，YI Wan-xian¹

(1. Institute of Urogenital System, Key Engineering Laboratory of Synthesis Biology, Shenzhen University, Shenzhen 518060; 2. Shenzhen Center for Disease Control and Prevention, Shenzhen 518055; 3. Key Laboratory of Optoelectronics Devices and Systems of Ministry of Education/Guangdong Province, College of Optoelectronics Engineering, Shenzhen University, Shenzhen 518060; 4. The First Affiliated Hospital of Shenzhen University, Shenzhen 518060, Guangdong, China)

Received:2013-09-12 Revised:2013-09-29 Online:2013-11-30 Published:2013-11-30
Contact: 梅树江，E-mail：sjmei@szcdc.net；WANG Xiao-mei， E-mail：xmwang@szu.edu.cn

Abstract

Abstract:

OBJECTIVE: The reproductive toxicity of static magnetic field (SMF) was investigated using mouse preantral follicle 3D culture in vitro system established in our laboratory. METHODS：Ovaries was removed from 12 days old female mice. (130±10) μm preantral follicles were isolated and encapsulated into alginate hydrogel，then cultured in droplets for 8 days. Experimental groups were treated by different intensity (5 and 450 mT) SMF. Diameter of follicles was measured every other day. The ovulation of antral follicles was induced by HCG hormone，and the matured oocytes (first polar body emission) were identified under the stereomicroscope. RESULTS：Follicles in each group were well developed on morphologically. Follicular diameter of each group gradually increased with culture time. Follicular diameter of 450 mT SMF-treated group was significantly increased at 4 days (P<0.05)，and diameter of 5 and 450 mT SMF- treated group was significantly increased at 6 days(P<0.05)，compared with that of control group. With the increasing intensity of SMF，the cavity formation rate of the follicles was obviously elevated，but the oocytes maturation rate of 450 mT SMF-treated group was significantly reduced compared with that of the control group. CONCLUSION： The alginate hydrogel could maintains the 3D structure of individual mouse follicle. With increasing strength of SMF，the diameter of secondary follicles was statistically increased，the cavity formation ratio was also markedly increased，but the process of oocyte maturation was inhibited by 450 mT SMF.

Key words: preantral follicle, 3D culture, oocyte maturation, static magnetic field

LIN Gui-miao，MEI Shu-jiang，LIN Su-xia，XU Gai-xia，LI Hui，ZHU Yue-quan，LIN Xiao-tan，CAI Zhi-ming，WANG Xiao-mei，MA Han-wu，LI Yuan，LU Yan，QIN Yan-min，YI Wan-xian. Effects of static magnetic field on proliferation of mouse preantral follicles 3D cultured in vitro[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.06.005.

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Effects of static magnetic field on proliferation of mouse preantral follicles 3D cultured in vitro

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