甲醛对HepG2细胞脂肪代谢的影响

doi:10.3969/j.issn.1004-616x.2013.06.003

Abstract

Abstract:

OBJECTIVE: To investigate the effects and possible mechanism of liquid formaldehyde on the triglyceride content of HepG2 hepatocytes. METHODS：HepG2 cells were treated with formaldehyde in 0.02，0.1，0.5，2.5 and 12.5 mmol/L concentrations，CCK-8 method was used to detect cell viability. Triglyceride was measured by triglyceride GPO-POD assay kit after formaldehyde exposure. The expression of carnitine palmitoyl transferase 1 (CPT1)，sterol regulatory element-binding protein (SREBP) 1c，ADP-ribosylation factor 1 (Arf1) and coat protein comlex Ⅰ(COPⅠ) were assessed by western blot after treatment with formaldehyde in 0.004，0.02，0.1 mmol/L concentrations. RESULTS：0.5－12.5 mmol/L FA exposure of 24 h or 0.1-12.5 mmol/L FA exposure of 48 h could significantly reduce the viability of HepG2 cells (P<0.05). TG contents were increased significantly after exposure to 0.1 mmol/L FA for 24 hours or 0.1 and 0.02 mmol/L FA for 48 h (P<0.05). CPT1 expression levels were significantly decreased after 48 h exposure，but SREBP-1c and COPⅠexpression levels were increased after both 24 h and 48 h exposures. Arf1 expression level was significantly increased after 24 h exposure. CONCLUSION：FA exposure could increase intracellular TG contents in HepG2 cells，and it may be associated with inhibition of fatty acid β oxidation and increased synthesis of triglyceride.

Key words: formaldehyde, triglyceride, CPT1, SREBP-1c, Arf1, COPⅠ

YAN Dan-dan，BAI Jian-ying，LIANG Rui-feng，WANG You-ping，LEI Pei-yu. Effects of formaldehyde on lipid metabolism in HepG2 cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.06.003.

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Effects of formaldehyde on lipid metabolism in HepG2 cells

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