应用LC/TOF-MS分析食管鳞状细胞癌血浆小分子代谢物的实验研究

doi:10.3969/j.issn.1004-616x.2013.06.002

Abstract

Abstract:

OBJECTIVE: The plasma low-molecular weight metabolites of esophageal squamous cell carcinoma were analyzed by liquid chromatography-time of flight- mass spectrography( LC/TOF-MS) to explore potential biomarkers for the early detection of ESCC. METHODS：Plasma samples were obtained from 30 ESCC patients and 30 healthy volunteers，low-molecular weight metabolites were identified by LC/TOF-MS，feature detection was performed by MassHunter software MFE algorithm，statistical analysis was conducted by MPP software，and primary identification was achieved by searching METLIN database. RESULTS：Compared to healthy controls，46 differentially expressed features were detected，13 of which were up-regulated in ESCC group，while the other 33 were down-regulated. After searching with METLIN database，plasma levels of PA，PS，PG were significantly lower in ESCC group. CONCLUSION：ESCC patients are markedly different from healthy people in plasma metabolite composition，and the LC/TOF-MS plasma metabonomics shed light on esophageal cancer early detection and screening.

Key words: metabonomics, liquid chromatography-time of flight-mass spectrography, low-molecular weight metabolites, esophageal squamous cell carcinoma

PENG Yuan，LIU Ran，LI Xiao-bo，YIN Li-hong. A pilot study of plasma metabolic biomarkers for esophageal squamous cell carcinoma by LC/TOF-MS[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.06.002.

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A pilot study of plasma metabolic biomarkers for esophageal squamous cell carcinoma by LC/TOF-MS

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