维生素E琥珀酸酯通过Akt/mTOR信号通路诱导人胃癌细胞SGC-7901发生保护性自噬

doi:10.3969/j.issn.1004-616x.2013.05.004

Abstract

Abstract:

OBJECTIVE: To investigate whether vitamin E succinate (VES) could induce autophagy in SGC-7901，and to explore the role of autophagy in cell proliferation inhibited by the VES. METHODS：Human gastric cancer cells SGC-7901 were treated with VES. Electron microscopy was used to study autophagosome morphology，and fluorescence microscopy for intracellular punctuate fluorescence of green fluorescence protein-LC3 (GFP-LC3). Western blot evaluated the level of autophagy marker protein LC3，and the activities of Akt and mTOR，the critical targets of Akt/mTOR signaling pathway. SGC-7901 cells were pre-treated with autophagy inhibitor，chloroquine (CQ) and 3-methyladenine (3-MA)，then we used MTT to detect the suppression of cell growth. RESULTS：Typical autophagic vesicles and autolysosome in VES treatment groups were identified. With increasing doses of VES，the punctuate fluorescence began to gather and intensity. LC3-II protein levels increased in cells treated with VES，suggested that VES may probably induce autophagy in SGC-7901. VES down-regulated the activities of the phosphory-Akt and phosphory-mTOR，suggested that the activity of Akt/mTOR signaling pathway was inhibited by VES. Combined action of VES+CQ group and VES+3-MA group significately suppressed cell growth compared with VES alone (P<0.05). When autophagy was inhibited ，the rate of cell proliferation was greatly reduced，suggesting that VES- induced autophagy had a protective effect on cell proliferation. CONCLUSION：Vitamin E succinate induced protective autophagy in human gastric cancer cells SGC-7901 via the Akt/mTOR signaling pathway.

Key words: vitamin E succinate, SGC-7901 gastric cancer cells, autophagy

HOU Li-ying，SUN Yan-pei，ZHANG Xu-guang，ZHAO Dong，WU Kun. Vitamin E succinate induced protective autophagy in human gastric cancer cells SGC-7901 via the Akt/mTOR signaling pathway[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.05.004.

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Vitamin E succinate induced protective autophagy in human gastric cancer cells SGC-7901 via the Akt/mTOR signaling pathway

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