三氧化二砷对膀胱癌T24细胞Apaf-1基因表达的影响

doi:10.3969/j.issn.1004-616x.2013.05.003

Abstract

Abstract:

OBJECTIVE: To observe the effects of arsenic trioxide (As2O3) on human bladder cancer T24 cell proliferation and apoptosis protease activating factor-1 (Apaf-1) gene expression. METHODS：T24 cells in the logarithmic growth phase，were digested with 0.25% trypsin into a cell density of 1×105/mL single cell suspension. Then cells were inculated with different concentrations (0，1，2，4，8 μmol/L) of As2O3，as well as blank control group for 24，48 and 72 h. Cell proliferation inhibition rate was evaluated by MTT. After cell treatment with As2O3 (0，1，2，4，8 μmol/L) for 72 h，deoxyribose situ terminal transferase labeling (TUNEL) method was used to measure apoptosis；RT-PCR and Western blotting to assess the impact of As2O3 on Apaf-1 mRNA and protein expression. RESULTS：Compared with the control group， the 2，4，8 μmol/L As2O3 dose groups could effectively inhibit the proliferation of T24 cells in a concentration-dependent manner (24 h，r =0.962，P=0.038；48 h，r =0.959，P=0.041；72 h，r =0.973，P=0.027). Cells treated with As2O3 at 1-8 μmol/L for 72 h， showed typical apoptotic changes in a concentration-dependent manner (r= 0.993，P=0.007); whilst Apaf-1 mRNA and protein expressions were also significantly increased in a concentration-dependent (mRNA，r =0.986，P=0.014；protein，r =0.989， P=0.000). CONCLUSION：As2O3 could effectively inhibit bladder cancer T24 cell growth，proliferation and induce apoptosis. The possible mechanism may be related to increased Apaf-1 gene expression.

Key words: arsenic trioxide, apoptotic protease activating factor-1, apoptosis, bladder tumor

SUN Jun-pei，LIU Jiu-hua，GU Heng，HUANG Jun-song，ZHANG Ming-cong. Effect of arsenic trioxide on Apaf-1 gene expression in bladder cancer T24 cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.05.003.

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Effect of arsenic trioxide on Apaf-1 gene expression in bladder cancer T24 cells

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