Abstract:

OBJECTIVE: To establish a new technique of multiple immunoenzyme histochemistry staining by using computer image processing technology. METHODS：We stained Hepatitis B virus surface protein (HBs)， asiologlycoprotein receptor (ASGPR) and proliferating cell nuclear antigen (PCNA) on the same hepatocellular carcinoma slice using the improved streptavidin-peroxidase method and combined these three figures into one that displayed the expression of these proteins together with the assistance of computer image processing technology. RESULTS：The positive signals of three proteins in the hepatocellular carcinoma slice were clear and strong without antibody cross reactivity. The precessed image could objectively display the exact site of positive signals in the original pictures and show the expression level of these different proteins together in one picture. CONCLUSION：This established method could mark multiple proteins in one slice together.

Key words: multiple staining, computer image processing technology, immunoenzyme histochemistry, eluted antibody