Prohibitin 3'-UTR 基因多态性与粤东乳腺癌发病风险的研究

doi:10.3969/j.issn.1004-616x.2013.04.011

Abstract

Abstract:

OBJECTIVE: To determine the association of Prohibitin (PHB) 3'- untranslated region (UTR) polymorphism with the risk of breast cancer in eastern Guangdong of southern China．METHODS：A case-control study of 231 breast cancer patients and 169 healthy controls were enrolled into this study. Genomic DNA was extracted from whole blood cells. Single nucleotide polymorphisms of rs1049620，rs9893420 and rs4987083 were determined by the high resolution melting (HRM) method．Data were analyzed via Logistic regression analysis and Chi-square test. RESULTS：PHB gene at rs9893420 and rs4987083 sites showed single genotype in breast cancer group and control. There were no significant differences in frequencies of G/G，A/G and A/A at rs1049620 between patients and controls(P>0.05). Meanwhile，no significant correlation could be found between rs1049620 SNP and clinico-pathologic characteristics of breast cancer such as histological classification and expression of hormone receptor. CONCLUSION： Our results do not lend support to the hypothesis that PHB polymorphism contributes to susceptibility of breast cancer.

Key words: breast cancer, Prohibitin, single nucleotide polymophism, high resolution melting

PENG Lin，HUANG Yi-teng，LUO Yu-hao. Prohibitin 3'-untranslated region polymorphism and breast cancer risk in eastern Guangdong of southern China[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.04.011.

References

[1] Nuell MJ，Stewart DA，Walker L，et al. Prohibitin，an evolutionarily conserved intracellular protein that blocks DNA synthesis in normal fibroblasts and HeLa cells[J]. Mol Cell Biol，1991，11 (3)：1372-1381.

[2] Jupe ER，Badgett AA，Neas BR. Single nucleotide polymorphism in prohibitin 3'untranslated region and breast-cancer susceptibility [J]. Lancet，2001，357 (9268)：1588–1589.

[3] 彭琳，陈建良，方裕森，等. Prohibitin rs6917 C>T基因多态性与潮汕女性乳腺癌易感性的关系研究[J]. 中国医药导报，2012，9 (31)：16-20

[4] DeSantis C，Siegel R，Bandi P，et al. Breast cancer statistics， 2011[J]. CA：Cancer J Clin，2011，61：409–418.

[5] Jupe ER，Liu XT，Kiehlbauch JL，et a1．Prohibitin in breast cancer cell lines：LOSS of antiproliferative activity is linked to 3' untranslated region mutations[J]．Cell Growth Differ，1996， 7 (7)：871-878．

[6] Spurdle AB，Hopper JL，Chen X，et al．Prohibitin 3'untranslated region polymorphism and breast cancer risk in Australian women[J]．Lancet，2002，360 (9337)：925-926．

[7] Campbell IG，Allen J，Eccles DM．Prohibitin 3'untranslated region polymorphism and breast cancer risk[J]．Cancer Epidemiol Biomarkers Prev，2003，12 (Sup 1)：1273-1274．

[8] Karakus N，Kara N，Ulusoy AN．Lack of association between Prohibitin 3'untranslated region C→T polymorphism and breast cancer in a Turkish population[J]．DNA Cell Biol，2008，27 (8)：449-452．

[9] Jakubowska A，Jaworska K，Cybulski C，et al．Do BRCA1 modifiers also affect the risk of breast cancer in non-carriers?[J]．Eur J Cancer，2009，45 (5)：837-842．

[10] Jakubowska A，Gmnwaid J，Grrski B，et al．The 3'untranslated region C> T polymorphism of prohibitin is a breast cancer risk modifier in Polish women carrying a BRCA1 mutation[J]．Breast Cancer Res Treat，2007，104 (1)：67-74．

[11] Zhou TB，Yin SS，Huang JJ，et al. Relationship between the prohibitin 3' untranslated region C > T gene polymorphism and cancer susceptibility ——results of a meta-analysis[J]. Asian Pac J Cancer Prev，2012，13 (7)：3319-3323.

[12] Weizmann Institute of Science. PHB Gene protein-coding GIFtS：65[DB/OL]. (2013-04-08) http://www.genecards.org/ cgi-bin/carddisp.pl?gene=PHB&snp=215#snp.

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Prohibitin 3'-untranslated region polymorphism and breast cancer risk in eastern Guangdong of southern China

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