Carcinogenesis, Teratogenesis & Mutagenesis

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Effect of curcumin on proliferation of human bile duct carcinoma MZ-Cha-1 cells

YANG Hai-bo,SONG Wei,CHEN Lan-ying*   

  1. College of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan 467036, Henan, China
  • Received:2012-11-09 Revised:2013-03-25 Online:2013-07-30 Published:2013-07-30
  • Contact: CHEN Lan-ying,E-mail:lychen666@163.com

Abstract:

OBJECTIVE: To study the apoptotic effects of human bile duct carcinoma MZ-Cha-1 cells induced by curcumin. METHODSAfter the MZ-Cha-1 cells induced by 0,10,20 and 40 μg/mL curcumin for 12,24,36 h,the apoptotic effects of curcumin were studied using the methods of MTT to detect the curcumin on MZ-Cha-1 cell suppression,and selected 10 μg/mL of curcumin treatment MZ-Cha-1 cells after 24 h,using Giemsa staining and Hoechst33258 dye to assess MZ-Cha-1 cell apoptosis,using flow cytometry to detect the cell cycle distribution. RESULTS:Compared with control group,the curcumin on MZ-Cha-1 bile duct carcinoma cell had obvious inhibitory effect,and in dose- and time- dependent mamer (P<0.05). Proliferation of MZ-Cha-1 cells could be inhibited by 10 μg/mL curcumin after 24 h. Light microscope showed that the morphology of the cells treated with curcumin appeared shrunken. Concentrated cell nucleus appeared granular and fluorescent by Hoechst33258 staining. Flow cytometry analysis showed that G0/G1 phase was increased and G2/M phase decreased (P<0.05). CONCLUSION:This study suggested that curcumin could induce apoptosis of the human MZ-Cha-1 cells effectively.

Key words: curcumin, human bile duct carcinoma, apoptosis