太白楤木皂苷的抗氧化作用研究

doi:10.3969/j.issn.1004-616x.2013.04.009

Abstract

Abstract:

OBJECTIVE: To evaluate the antioxidative effect of saponin from Aralia taibaiensis，and provide some basic data for its further application in the prevention and treatment for oxidative stress-related diseases. METHODS： The total antioxidant capacity of saponin was determined by ferric-reducing ability of plasma (FRAP) method. In the cellular study，tert-butyl hydroperoxide (tBHP) was employed as an oxidant to induce oxidative stress，and saponin (5 μg/mL) was pre-incubated with the cells 12 h before challenged by tBHP in the protective group. Then cellular ROS was labeled with DCFH-DA and determined by flow cytometry. The protein levels of nuclear respiratory factor 2 (Nrf2)，heme oxygenase-1 (HO-1)，γ-glutamate-cysteine ligase (GCL) were evaluated by Western blot. RESULTS：The saponin showed marked antioxidative effect. tBHP induced massive reactive oxygen species (ROS) production，for which saponin pre-treatment showed significant inhibition. Saponin pre-treatment also significantly inhibited the tBHP-stimulated protein expressions of Nrf2，HO-1 and GCL. CONCLUSION：Saponin from Aralia taibaiensis has potent antioxidative activity，indicating its potential clinical application in oxidative stress-related diseases.

Key words: Aralia taibaiensis, antioxidative, reactive oxygen species, saponin, nuclear respiratory factor 2

GUO Yu，YANG Pei，ZHOU Xue-ying，HAI Chun-xu，QIN Xu-jun，LI Jin-gang. The antioxidative activity of saponin from Aralia taibaiensis[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.04.009.

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The antioxidative activity of saponin from Aralia taibaiensis

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