FOLFOX4与1-D-甲基色氨酸联合治疗对荷胃癌小鼠调节性T细胞的影响

doi:10.3969/j.issn.1004-616x.2013.04.006

Abstract

Abstract:

OBJECTIVE: To investigate whether combining 1-D-MT with FOLFOX4 could be useful to improve the immune tolerance of gastric cancer- bearing mice. METHODS：By using the lipofectamineTM 2000，the eukaryotic expression plasmid pcDNA3.1-IDO and empty vector pcDNA3.1 (+) were transfected in a MFC cell line，setting a control group. The expression of IDO was detected by reverse transcription polymerase chain reaction (RT-PCR) and western blot. Animal model of gastric cancer- bearing mice were established to receive normal saline (NS)，FOLFOX4，1-D-MT and 1-D-MT+FOLFOX4 therapy. By using flow cytometry，Treg cell ratio change was analyzed and FOXP3 mRNA expression change was assessed by RT-PCR. RESULTS：The expression of IDO mRNA and protein in the group trancfected with pcDNA3.1-IDO was obviousely ligher than the MFC group and the empty vector group (P<0.05). Treg cell ratio and FOXP3 mRNA expression in the transfected IDO negative control group with NS therapy was higher than the MFC and the empty vector groups (P<0.05). The Treg ratio and FOXP3 mRNA in 1-D-MT+FOLFOX4 and 1-D-MT groups were less than in FOLFOX4 group (P<0.05)，with 1-D-MT+FOLFOX4 group more markedly than 1-D-MT group (P<0.05). CONCLUSION：Combining 1-D-MT with FOLFOX4 could reduce Treg cell ratio and FOXP3 expression，thus reducing the immune escape.

Key words: 1-D-methyl tryptophan, FOLFOX4, gastric cancer, regulatory T cell

KANG Gui-yun，LI Zhe-ping，LIU Xiao-li，WU Xi-run，SHEN Hui-qin，WANG Qi. The effect of combining FOLFOX4 with 1-D-methyl tryptophan on regulatory T cells of gastric cancer-bearing mice[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.04.006.

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The effect of combining FOLFOX4 with 1-D-methyl tryptophan on regulatory T cells of gastric cancer-bearing mice

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