芹菜素对非洲爪蟾卵母细胞体外成熟的影响及其作用机制

doi:10.3969/j.issn.1004-616x.2013.04.003

Carcinogenesis, Teratogenesis & Mutagenesis

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Effect and mechanism of apigenin on in vitro maturation of Xenopus oocytes

ZHANG Lun，GAO Shu，PENG Zhi-qing，HOU Li-ying，WU Kun*

Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, Harbin 150081, Heilongjiang, China

Received:2013-03-26 Revised:2013-05-30 Online:2013-07-30 Published:2013-07-30
Contact: WU Kun，E-mail：wukun_15000@126.com

Abstract

Abstract:

OBJECTIVE: To explore the effect and mechanism of different doses of apigenin on in vitro maturation of Xenopus oocytes. METHODS：Healthy Xenopus oocytes were cultured in different doses (20，80，160 and 500 μmol/L) of apigenin. Meanwhile negative and positive control groups were set. Then we observed and recorded the rate of germinal vesicle breakdown (GVBD) per hour，confirmed the transformation of cell nucleus and chromosome by means of trichloroacetic acid fixation and fluorescence staining. Finally oocytes were collected at 8 h in each group，and the expressions of p-Erk1，Mos and CyclinB2 were detected by Western blot. RESULTS：Compared with negative control group，GVBD rates were all highly increased when apigenin-treated dose were 80，160 and 500 μmol/L，and time were 4-8 h (P<0.05). The expressions of p-Erk1，Mos and CyclinB2 were all markedly increased when the dose of apigenin were between 80 and 500 μmol/L (P<0.05). CONCLUSION：Apigenin may play an important role in promoting the maturation of Xenopus oocytes in vitro through regulation of mature promoting factor (MPF) and mitogen-activated protein kinase (MAKP).

Key words: apigenin, oocytes, in vitro maturation, Xenopus

ZHANG Lun，GAO Shu，PENG Zhi-qing，HOU Li-ying，WU Kun. Effect and mechanism of apigenin on in vitro maturation of Xenopus oocytes[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.04.003.

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Effect and mechanism of apigenin on in vitro maturation of Xenopus oocytes

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