松花江水中有机提取物对MCF-7细胞增殖、迁移及对Bcl-2/Bax蛋白表达的影响

doi:10.3969/j.issn.1004-616x.2013.03.004

Abstract

Abstract:

OBJECTIVE: Exploring the environmental estrogenic effect and mechanism of cell proliferation induced by organic extracts from Songhua River. METHODS：1 000 L water sample was collected from upstream water (Sifangtai source water)of Songhua River in March 2011. Organic substances were extracted by GDX-102 resin. MCF-7 cells were cultured with different concentrations of organics extracts (the concentrations were equivalent to those from 25，100，400，1 600 and 3 200 mL of river water). We measured estrogenic effect by MTT assay and investigated invasion and migration of MCF-7 cells by wound healing assay and transwell assay. We also explored the influence of organic extracts on Bcl-2 and Bax protein expressions by immunocytochemical method. RESULTS：The estrogenic effect was gradually enhanced with increasing concentration of organic extracts，significantly different compared to negative control group (P<0.05). The estrogenic effect and promotion of cell invasion and migration were the strongest when the content of organic extracts was equivalent to 1 600 mL of river water. Both Bcl-2 and Bax proteins could be expressed in MCF-7 cells. Comparing to negative control，with increasing concentration of organic extracts， the expression of Bcl-2 protein was gradually increased while that of Bax protein decreased. CONCLUSION：Organic extracts from upstream water(Sifangtai source water) of Songhua River could promote the proliferation of MCF-7 cells by regulating the ratio of Bcl-2 and Bax proteins，and stimulate invasion and migration of MCF-7 cells.

Key words: water, organic extracts, estrogen, MCF-7 cells

LIAO Yuan-hai，WANG Yan-mei，DONG Shu-ying，WANG Yang. The influence of organic extracts in Songhua River on proliferation and migration of MCF-7 cell as well as expression of Bcl-2/Bax protein[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.03.004.

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The influence of organic extracts in Songhua River on proliferation and migration of MCF-7 cell as well as expression of Bcl-2/Bax protein

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