γ射线照射诱导胸腺中调节性T细胞表型改变的研究

doi:10.3969/j.issn.1004-616x.2013.03.003

Abstract

Abstract:

OBJECTIVE: To analyze the radiation-influence induced changes of the number and phenotype of the thymocytes and its regulatory subsets in mice. METHODS：Mice were exposed to 2 Gy 60Co γ-ray in a whole body manner，and thymocytes were separated and counted in 1，4 and 10 d after irradiation. The percentage of CD4+ T and reg in thymocytes and the expression of CD39，CD103 in Treg were analyzed using FACS. RESULTS：There were differences in time-response to radiation. The thymocyte and CD4SP T cell numbers were decreased significantly after whole body irradiation and reached a minimum in 4 days，but recovered clearly after 10 days. However the Treg cell decreased in a manner of non-statistical difference in 4 days，but decreased significantly in 10 days. The percentage of Treg increased first then decreased in a time-response manner. The percentage of CD39+ Treg cell and MFI of CD39 were increased after 4 days’ irradiation. The percentage of CD103+ Treg cell was also increased，however its MFI was deceased after 4 days’ irradiation. CONCLUSION：Treg cells were more resistant to radiation than other thymocytes，resulting in the immune suppression of whole body. The expressions of CD39 and CD103 were differentially regulated by radiation and may play an important role in mediating suppressive function of Treg.

Key words: ionizing radiation, regulatory T cell, phenotype, thymus

LIU Lan-tao，LIU Jian-xiang，SU Xu. Phenotypic changes of regulatory T cells in thymus induced by ionizing radiation[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.03.003.

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Phenotypic changes of regulatory T cells in thymus induced by ionizing radiation

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