Abstract:

OBJECTIVE: To evaluate genetic toxicity of transgenic human α-lactalbumin powdered milk. METHODS：In Ames test，TA97，TA98，TA100，and TA102 strains were treated by 62，185，556，1 667 and 5 000 μg transgenic human α-lactalbumin powdered milk per plate. In mice bone marrow cell micronucleus test and mice sperm abnormality test，2.5，5.0 and 10.0 g/kg transgenic human α-lactalbumin powdered milk groups，CP positive and water negative control groups were set up. RESULTS：Back mutation colonies of transgenic human α-lactalbumin powdered milk groups did not exceed twice that of the control in Ames test and there was no dose-response relationship. In mice bone marrow cell micronucleus test，no significant difference was found in PCE/RBC and micronucleus rate between transgenic human α-lactalbumin powdered milk-treated groups and negative control group. In mice sperm abnormality test，sperm abnormality rate of transgenic human α-lactalbumin powdered milk-treated groups was lower than that of negative control. CONCLUSION：No genetic toxicity was observed in transgenic human α-lactalbumin powdered milk.

Key words: transgenic powdered milk, α-lactalbumin, recombinant protein, genetic toxicity, Ames test, micronucleus test, sperm abnormality test