Carcinogenesis, Teratogenesis & Mutagenesis

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The identification of MARDHRS4 and its regulation on DHRS4 transcription

CHANG Xiao-lan1，FENG Lei1，FENG Guang-you2，*   

  1. 1. Department of Cell Biology, Shantou University Medical College, Shantou 515041; 2. Department of Pathophysiology, Shantou University Medical College, Shantou 515041, Guangdong, China
  • Received:2012-10-10 Revised:2013-01-07 Online:2013-03-30 Published:2013-03-30
  • Contact: FENG Guang-you，E-mail：gyfeng@stu.edu.cn

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Abstract:

OBJECTIVE: To screen the novel antisense transcriptions of DHRS4 and study their functions. METHODS：RACE was used to clone the antisense transcription of DHRS4 from the NCTC1469 cells (normal mouse liver cell line). Based on the antisense transcription cloned above，the suitable siRNA was designed and transfected the NCTC1469 cells. 36 h after successful transfection，the relative amount of DHRS4 mRNA in transfected cells was tested by RT-qPCR. RESULTS：A novel 835 nt natural antisense RNA was successfully cloned and identified. It belonged to a kind of LncRNA and was named MARDHRS4 (mouse antisense RNA of DHRS4). DHRS4 mRNA decreased about 40% after interruption of siRNA to MARDHRS. CONCLUSION：MARDHRS4，one kind of LncRNA transcripted from the opposite strand of DHRS4，may play a role in enhancing transcription of DHRS4.

Key words: MARDHRS4, DHRS4, long non-coding RNA, transcription