应用MALDI-TOF MS 检测5种白血病细胞株DCK和CDA基因部分单核苷酸多态性

doi:10.3969/j.issn.1004-616x.2013.02.008

Carcinogenesis, Teratogenesis & Mutagenesis

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Detection of SNPs of DCK and CDA genes in five leukemia cell lines with MALDI-TOF MS

ZHANG Xiao-ping¹，JIAN Zi-ying¹，CHEN Bao-an^1，*，XU Pei-pei¹，PENG Miao-xin¹，XIA Guo-hua¹，CHENG Lu²，LU Zu-hong²

1. Department of Hematology, Zhongda Hospital, Key Subject of Jiangsu Province, Southeast University Medical College, Nanjing 210009; 2. National Key Laboratory for Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China

Received:2012-05-15 Revised:2012-12-15 Online:2013-03-30 Published:2013-03-30
Contact: CHEN Bao-an，E-mail：cba8888@hotmail.com

Abstract

Abstract:

OBJECTIVE: This study was aimed to detect single nucleotide polymorphisms (SNPs) of deoxycytidine kinase(DCK) gene，cytidine deaminase(CDA) gene in five different leukemia cell lines. METHODS：Cell line K562 Ka HL-60 U937 Raji were cultured. The genomic DNA was isolated by QIAamp DNA Blood Mini Kit. Designed primers were amplified by PCR，using the related DNA fragments. DCK gene A674G(rs111454937) C1644T (rs72552079)，CDA gene A79C(rs2072671) G208A(rs60369023) was genotyped by means of matrix assisted laser desorption ionisation-time of flight mass spectrometry method (MALDI-TOF MS). RESULTS：The genotype of locus A674G (rs111454937) on DCK gene in all five leukemia cell lines was A/A. The genotype of locus C1644T (rs72552079) was C/C. The genotype of locus G208A(rs60369023) on CDA gene in all five hematology system cell lines was G/G. For HL-60，U937，Raji cell lines，the genotype of locus A79C(rs2072671) on CDA gene was A/A，while for K562，Ka cell lines it was C/A. CONCLUSION：The genotype of locus A79C(rs2072671) on CDA gene was different in the 5 tested cell lines of hematological malignancy. The genotypes of other 3 loci were the same in all 5 cell lines.

Key words: DCK gene, CDA gene, single nucleotide polymorphisms, leukemia

ZHANG Xiao-ping1，JIAN Zi-ying1，CHEN Bao-an，XU Pei-pei，PENG Miao-xin，XIA Guo-hua，CHENG Lu，LU Zu-hong. Detection of SNPs of DCK and CDA genes in five leukemia cell lines with MALDI-TOF MS[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.02.008.

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Detection of SNPs of DCK and CDA genes in five leukemia cell lines with MALDI-TOF MS

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