Hh信号通路在口腔鳞状细胞癌中的激活及意义

doi:10.3969/j.issn.1004-616x.2013.02.006

Abstract

Abstract:

OBJECTIVE: To examine the expressions of Shh，Gli1 and Ptch，the key elements of Hedgehog signaling pathway in oral squamous cell carcinoma and normal oral mucosa，and to explore the relationship between their expressions and biological parameters. METHODS：The expressions of Shh，Ptch，Gli1 were examined in 40 cases of oral squamous cell carcinoma and 30 normal oral mucosa (as control) by immunohistochemistry. The expressions of Shh，Gli1，Ptch mRNA were analyzed in specimens from 10 oral squamous cell carcinoma and 5 nomal oral specimens by RT-PCR . RESULTS：There was no expression of Shh，Ptch，Gli1 in 30 specimens of normal oral mucosa. The positive expression rates of Shh，Ptch，Gli-1 in oral squamous cell carcinoma were 62.5%，60.0%，65.0%，respectively. Nonparametric Mann-Whitney test showed that the expressions of Shh and Gli-1 were related to the tumor size，lymph node metastasis and clinical stages (P<0.05). But the expression of Ptch was related to metastasis (P<0.05). A statistically significant correlation was found among the expressions of Shh，Ptch and Gli-1 (rs=0.527，0.406，0.578，respectively， P<0.01). Shh，Ptch and Gli-1 mRNA in 60%，50%，70%，respectively，of oral squamous cell carcinoma were higher than in nomal oral mucosa，the difference was statistically significant (P<0.05). CONCLUSION：The activation of hedgehog signaling pathway in oral squamous cell carcinoma and hedgehog signaling pathway may be related to the development and metastasis of oral squamous cell carcinoma.

Key words: oral squamous cell carcinoma, Sonic hedgehog, Patched, Gli-1

WANG Jun-ju，SONG Ling-gang，ZHANG Kai，LIU Yan-ping. The activation and function of Hh signaling pathway in oral squamous cell carcinoma[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.02.006.

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The activation and function of Hh signaling pathway in oral squamous cell carcinoma

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