四物汤抗环磷酰胺诱变作用的研究

doi:10.3969/j.issn.1004-616x.2013.01.013

Abstract

Abstract:

OBJECTIVE: To study the anti- mutagenic effects of Siwu decoction. METHODS：Four test methods were used：Mouse bone marrow cell micronucleus test，chromosomal aberrations in mouse bone marrow cells test，micronucleus test and chromosomal aberration test in human peripheral blood lymphocytes in vitro. Five groups were included in each test method，including blank control group (physiological saline group)，positive control group (cyclophosphamide group) and different doses of Siwu decoction groups. High dose group Siwu decoction was 9.36 mg/(g·d)， middle dose group 4.68 mg/(g·d)，and low dose group 2.34 mg/(g·d). In blood lymphocytes in vitro test，Siwu decoction was added to culture medium in the form of serum. To prepare serum in rabbits，high dose was 3.36 g/ (kg·d)，middle dose 1.68 g/ (kg·d) and low dose 0.84 g/(g·d). Cells contained micronucleus and chromosomal aberrations in specimens were examined and counted. RESULTS：Compared with positive control group，the rate of micronucleus and chromosomal aberrations in three different Siwu decoction dose groups were obviously lowered (P＜0.01). Compared with blank control group，the rate of micronucleus and chromosomal aberrations in three different Siwu decoction dose groups were higher (P＞0.05). CONCLUSION：In the range of this experiment， Siwu decoction showed obvious anti-mutagenic effects.

Key words: Siwu decoction, anti-mutagen, micronucleus, chromosomal aberration

DONG Xiao-yan. The anti- mutagenic effects of Siwu decoction[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.01.013.

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The anti- mutagenic effects of Siwu decoction

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