新疆哈萨克族食管癌survivin启动子区CpG岛甲基化状态及其临床意义

doi:10.3969/j.issn.1004-616x.2013.01.009

Abstract

Abstract:

OBJECTIVE: To study the different expressions of survivin gene in esophageal cancer (EC) tissue，then explore their promoter CpG island methylation state，and analyze the change and involvement of promoter methylation on gene expression in Xinjiang Kazak's EC. METHODS：Using RT-PCR to detect the mRNA expression of survivin，and MSP to assess the methylation state of survivin CpG island in promoter region in EC tissues and distal non-cancerous tissues. RESULTS：In the 20 pairs of EC tissues，survivin mRNA positive ratio in EC tissues (95%) was higher than distal non-cancerous tissues (65%) (P＜0.05). The methylation of CpG islands were mostly heterozygous type in both EC and non-cancerous tissues，and there were no significant difference between them (P>0.05). CONCLUSION： High expression of survivin was involved in the apoptosis and cell cycle regulation in the development of EC. But the expression display no relation with the CpG methylation state，suggesting that the latter did not regulate survivin expression directly in the development of EC.

Key words: Kazakh’s esophageal carcinoma, survivin；RT-PCR, methylation specific PCR, methylation

LIU Ling，LI Hui，WANG Hong-jiang，PANG Zuo-liang，ZHANG Rong-xiu，LI Hui-wu. The clinical significance and methylation state of survivin promoter CpG island in Kazak's esophageal cancer[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.01.009.

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The clinical significance and methylation state of survivin promoter CpG island in Kazak's esophageal cancer

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