Carcinogenesis, Teratogenesis & Mutagenesis

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Combined toxic effect of chorpyriphos and dimethoate on cholinesterase in rats

WU Hui,YI Nan-nan,LI Ting-ting,HE Xian-song,ZHAO Min-xian,YAO Xin-ya,WANG Can-nan*   

  1. (Key Laboratory of Medical Engineering Environment, Nutrition and Food Hygiene Department, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, China)
  • Received:2012-09-19 Revised:2012-11-28 Online:2013-01-30 Published:2013-01-30
  • Contact: WANG Can-nan,E-mail:wcnseu@126.com

Abstract:

OBJECTIVE: To study the combined toxic effect of chorpyriphos and dimethoate on plasma and brain cholinesterase activities in female rats. METHODS3×3 factorial analysis was used in this experiment. Adult female SD rats were randomly divided into 10 groups of ten rats (including a control group and nine treated groups) according to their weight. The NOAEL (no observed adverse effect level),1/100 LD50 (median lethal dose)and 1/50 LD50 of chlorpyrifos and dimethoate were selected as the low,medium,and high dose level,respectively. The dose of chlorpyrifos were 0.03,1.35,2.70 mg/ (kg·d) respectively,and those of dimethoate were 1.20,3.25,6.50 mg/ (kg·d) respectively. Blood was collected through arteria cruralis after 30 days of treatment via gastric gavage,then rats were killed and tissues including cortex and hippocampus were collected. Cholinesterase activity was estimated by the Pureauto CHE. RESULTS:The difference of ChE activity between high dose group and control group was significant. No interaction was noted between chorpyriphos and dimethoate on plasma and cortex AChE. The combination of chorpyriphos and dimethoate caused an interactive effect on plasma and brain BuChE inhibition and hippocampus AChE inhibition (P<0.01). CONCLUSION:High dose combination of organophosphorus pesticides could significantly inhibit the activity of ChE in blood and brain,and also lead to an interactive effect on plasma and brain ChE inhibition.

Key words: chorpyriphos, dimethoate, acetylcholinesterase, butyrylcholinesterase, combined effect, factorial analysis