叶酸和核黄素对乳腺癌患者淋巴细胞8和17号染色体非整倍体的影响

doi:10.3969/j.issn.1004-616x.2013.01.006

Abstract

Abstract:

OBJECTIVE: The research was to test the effect of folate (FA) and riboflavin (RF) on chromosome 8 and 17 aneuploidy in lymphocytes of breast cancer patients and normal controls. METHODS：Lymphocytes were cultured in each of the four media：high (200 nmol/L，HF) and low (20 nmol/L，LF) FA，high (500 nmol/L，HR) and low (1 nmol/L，LR) RF media (LFLR，LFHR，HFLR，HFHR). Chromosomes 8 and 17 aneuploidy was measured in mononucleated (MONO) and cytokinesis-blocked binucleated (BN) cells using dual-color ?uorescence in situ hybridization (FISH). RESULTS：In lymphocytes of breast cancer subjects and controls，LF media (LFLR or LFHR) induced significant and similar increases in frequencies of aneuploidy of chromosomes 8 and 17 relative to culture in HF media (HFLR or HFHR) (P<0.01). The frequency of 8 and 17 aneuploidy in breast cancer subjects was significantly higher than those of normal controls (P<0.01). FA and breast cancer status had significant impact on the frequency of aneuploidy (P<0.01)，while effect of FA was stronger deficiency. RF deficiency partially affected chromosome segregation，but not significantly (P>0.05). CONCLUSION：FA deficiency is a possible cause of chromosome 8 and 17 aneuploidy. FA is the dominant factor that affects chromosome segregation in our experimental system.

Key words: folate, riboflavin, aneuploidy, FISH

NI Juan，ZOU Tian-ning，WANG Xu. The effect of folate and riboflavin on chromosomes 8 and 17 aneuploidy in lymphocytes of breast cancer patients and normal controls[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.01.006.

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The effect of folate and riboflavin on chromosomes 8 and 17 aneuploidy in lymphocytes of breast cancer patients and normal controls

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