电离辐射对人外周血线粒体编码基因mRNA表达的影响

doi:10.3969/j.issn.1004-616x.2013.01.005

Abstract

Abstract:

OBJECTIVE: To explore the effect of ionizing radiation on mitochondrial gene expression in human peripheral blood cells. METHODS：Human peripheral blood samples were exposed to 60Co γ-ray at different doses，and its RNA was isolated from the white cells. The relative real-time quantitative PCR by standard curve method was set up using β-actin as internal standards. With this method，ND1，ND6，COXI，COXII，COXIII，ATPase6， ATPase8 gene expression changes after ionizing radiation were recorded，the relationship between mitochondrial gene expression and dose was analyzed by software of origin 7.5. RESULTS：The gene expression of COXI and ATPase6 after radiation of peripheral blood cells increased with the irradiation doses. From 0-3 Gy range，the relationship between the ratio of COXI mRNA/β-actin could be represented as：Y=0.625 9+0.256 1 D (r2=0.916 3，P<0.01)，the ATPase6 mRNA/ β-actin was：Y=0.218 9+ 0.806 0 D (r2=0.912 6，P<0.01). The relationship between the ratio of other mRNA/β-actin was not statistically different (P>0.05). CONCLUSION：Differential expression of COXI，ATPase6 mRNA was induced by ionizing radiation，so COXI and ATPase6 could be promising radiation biological dosimeters.

Key words: ionizing radiation, mitochondrial, gene expression, peripheral blood

ZHANG Zhong-xin，LIU Jian-gong，ZHANG Shu-xian， DUAN Zhi-kai. Effects of radiation on mitochondrial gene expression in human peripheral blood cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.01.005.

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Effects of radiation on mitochondrial gene expression in human peripheral blood cells

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