GMA致人支气管上皮细胞恶性转化过程中抑癌基因P15 甲基化状态的研究

doi:10.3969/j.issn.1004-616x.2013.01.004

Abstract

Abstract:

OBJECTIVE: Analyzing the changes of P15 methylation status among different stages of malignant transformation of human bronchial epithelial cells (16HBE) induced by glycidyl methacrylate (GMA) and discussing the role of DNA methylation in the process. METHODS: Cells were harvested at different times，protophase (the 10th generation)，metaphase (the 20th generation) and anaphase (the 30th generation) of transformed cells. Then these methylation statuses of P15 were detected by methylation chip and methylation-specific PCR (MSP)，and compared to the control groups (treated with DMSO) of the same generation. RESULTS：Based on the result of methylation chip and MSP，the methylation of P15 gene in 16HBE cells transformed by GMA occurred in both metaphase and anaphase but not in protophase. CONCLUSION：Gene of P15 could be regarded as a specific gene related to the degree of malignancy and a particular mutation biomarker of cell malignant transformation.

Key words: glycidyl methacrylate, human bronchial epithelial cells, malignant transformation, methylation

LI Huan-huan1，WANG An-na1，WANG Quan-kai，TAN Feng，XU Jian-ning. Study on the anti-oncogene of P15 methylation status in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.01.004.

References

[1] Dodge JE，Ramsahoye BH，Wo ZG，et al. De novo methylation of MMLV provirus in embryonic stem cells：CpG versus non-CpG methylation[J]. Gene，2002，289 (1/2)：41-48.

[2] Ndlovu MN，Denis H，Fuks F. Exposing the DNA methylome iceberg[J]. Trends Biochem Sci，2011，36 (7)：381-387.

[3] Wong E，Wei CL. Genome-wide distribution of DNA methylation at single-nucleotide resolution[J]. Prog Mol Biol Transl Sci， 2011，101：459-477.

[4] 董琳，李瑛，王全凯，等. 甲基丙烯酸环氧丙酯致人支气管上皮恶性转化细胞DNA修复基因点突变的研究[J]. 癌变·畸变·突变，2009，21 (1)：1-5.

[5] 杨敏，许建宁，王全凯，等. 甲基丙烯酸环氧丙酯致人支气管上皮细胞恶性转化研究[J]. 中华预防医学杂志，2009，43 (3)：187-192.

[6] Herman JG，Graf f JR，Myohanen S，et al. Methylation-specific PCR：A novel PCR assay for methylation status of CpG islands [J]. Proc Natl Acad Sci USA，1996，93 (18)：9821-9826.

[7] Rao PV，Gupta N，Bhaskar AS，et al. Toxins and bioactive compounds from cyanobacteria and their implications on human health[J]. J Enbiron Biol，2002，23 (3)：215-224.

[8] Herman JG，Jen J，Merlo A，et al. Hypermethylation-associated inactivation indicates a tumor suppressor Role for p15INK4BI[J]. Cancer Res，1996，56 (4)：722-727.

[9] Lubbert M. Gene silencing of the p15/INK4B cell-cycle inhibitor by hypermethylation：an early or later epigenetic alteration in myelodysplastic syndromes?[J]. Leukemia，2003，17 (9)： 1762- 1764.

[10] 周涛，陆红，范洪涛. 非霍奇金淋巴瘤基因甲基化及去甲基化再表达的研究[J]. 肿瘤杂志，2005，20 (2)：154-156.

[11] Bender CM，Pao MM，Jones PA. Inhibition of DNA methylation by 5-Aza-I-deoxycytidine suppresses the growth of human tumor- cell lines [J]. Cancer Res，1998，58 (1)：95-101.

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Study on the anti-oncogene of P15 methylation status in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate

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