Abstract:

OBJECTIVE: To explore the inhibitory effect of 131I-monoclonal antibody on tumor growth of human ovarian cancer cell line OC-3-VGH in nude mice. METHODS：The transplanted human ovarian carcinoma model was established by subcutaneous injection of OC-3-VGH cells in nude mice. The mice were randomly divided into negative control group，CP positive control group (60 mg/kg)，high dose (10 mg/kg)，low dose (2 mg/kg) group of monoclonal antibody，high dose (10 mg/kg＋125 μCi)，medium dose (6 mg/kg＋75 μCi)，low dose (2 mg/kg＋25 μCi) group of 131I- monoclonal antibody. Seven groups received continuously intraperitoneal injection for 14 d. Diameters of tumors were measured and nude mice were weighed on d0，d4，d8，d12，d15. The animals were killed 24 h after the last treatment. The transplanted tumors were weighed，the inhibitory rate and treatment over control growth ratios were calculated. RESULTS：The high dose monoclonal antibody group，and medium and high dose 131I-monoclonal antibody groups had significant tumor inhibiting effects in vivo，with treatment over control growth ratios of 54%，48%，30% and inhibitory rates of 33.59%，45.80% and 64.89%，respectively. Compared with the negative control，there was significant difference(P＜0.01) . The high dose monoclonal antibody group was compared with the high dose 131I-monoclonal antibody group，showing a statistical difference (P＜0.05). CONCLUSION： Both monoclonal antibody and 131I- monoclonal antibody groups effectively inhibited the growth of human ovarian carcinoma in vivo. The therapeutic efficiency was enhanced significantly in the high dose 131I-monoclonal antibody group.

Key words: nude mice, ovarian cancer, transplanted tumor, tumor inhibitory rate, radioimmunotherapy