浊漳河水体中有机污染物的遗传毒性研究

doi:10.3969/j.issn.1004-616x.2012.06.016

Carcinogenesis, Teratogenesis & Mutagenesis

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Study on genotoxicity of organic pollutants in the Zhuozhang River

LIU Rui－xiang1, REN Jia－hong1,2, CHANG Hui－li3, HUO Zhuan－zhuan1, LIU Tong1, YAN Ke－feng1

1. Department of Biology Science and Technology, Changzhi College, Changzhi 046011, Shanxi; 2. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, Jiangsu; 3. Environmental Monitoring Station of Changzhi, Changzhi 046000, Shanxi, China

Received:2011-10-21 Revised:2012-04-07 Online:2011-11-30 Published:2011-11-30
Contact: LIU Rui－xiang，E-mail：ruixiangliu1964@163.com
About author:刘瑞祥(1964- )，男，汉族，硕士，研究方向：遗传毒理学。E-mail：ruixiangliu1964@163.com
Supported by:
山西省高校科技研究开发基金资助项目(20081043)

Abstract

Abstract:

OBJECTIVE: The genotoxicity on human peripheral lymphocytes by organic pollutants in the Zhuozhang River was investigated. METHODS：The comet assay was conducted to detect DNA damage induced by the organic extracts of three typical sample sites. RESULTS：Within certain concentrations，the DNA damage in human peripheral lymphocytes was induced by the substances tested and increased with the dose of the organic extracts. Compared with the negative control group，there were significant differences(P<0.01). The differences of DNA damage were also shown in different samples by multiplex comparison at the dose of 100 ml/tube，with decreasing activity as follows，Baoma>Huangnian>Shiliang. CONCLUSION：The organic pollutants in Zhuozhang River caused DNA damage in human peripheral blood lymphocytes. This study also demonstrated that comet assay could be successfully applied to the genotoxicity monitoring of water organic extracts.

Key words: comet assay, DNA damage；organic pollutants, human peripheral lymphocytes

LIU Rui－xiang1, REN Jia－hong1,2, CHANG Hui－li3, HUO Zhuan－zhuan1, LIU Tong1, YAN Ke－feng1. Study on genotoxicity of organic pollutants in the Zhuozhang River[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2012.06.016.

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Study on genotoxicity of organic pollutants in the Zhuozhang River

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