ERCC2/XPD基因缺失对苯并[a]芘所致DNA损伤修复的影响

doi:10.3969/j.issn.1004-616x.2012.06.001

Carcinogenesis, Teratogenesis & Mutagenesis

Effect of deficient ERCC2/XPD gene on the repair of DNA damage induced by benzo[a]pyrene

XIAO Sha，LIU Qiu-fang，XU Tao-jun，GUAN Yang-yang，JIN Cui-hong，LI Dan-dan，LU Xiao-bo*

Hygiene Toxicology Department, Public Health School, China Medical University, Shenyang 110001, China

Received:2012-07-16 Revised:2012-09-25 Online:2011-11-30 Published:2011-11-30
Contact: LU Xiao-bo，E-mail：xblu@mail.cmu.edu.cn
About author:肖莎 (1986- )，女，湖北人，硕士研究生，研究方向：遗传毒理学。
Supported by:
国家自然科学基金 (30972506)

Abstract

Abstract:

OBJECTIVE: To investigate ERCC2/XPD (excision repair cross complementation group 2/Xeroderma pigmentosum D) function in the repair of DNA damage induced by benzo[a]pyrene. METHODS：China hamster ovary (CHO) cell line including wild type AA8 and ERCC2/XPD defective mutant UV5，was set up as a cell contrast model. DNA damage levels at different time points after benzo[a]pyrene treatment were evaluated by MTT cell inhibition assay，modified comet assay and Rad51 immunofluorescence test. RESULTS：UV5 was more sensitive to benzo[a]pyrene when compared with AA8. The DNA damage caused by benzo[a]pyrene was repaired by AA8 but not by UV5. CONCLUSION：ERCC2/XPD，as an important helicase in nucleotide excision repair，could play a critical role in repairing DNA damage induced by benzo[a]pyrene.

Key words: ERCC2/XPD, benzo[a]pyrene, nucleotide excision repair, AA8, UV5

XIAO Sha，LIU Qiu-fang，XU Tao-jun，GUAN Yang-yang，JIN Cui-hong，LI Dan-dan，LU Xiao-bo*. Effect of deficient ERCC2/XPD gene on the repair of DNA damage induced by benzo[a]pyrene[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2012.06.001.

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Effect of deficient ERCC2/XPD gene on the repair of DNA damage induced by benzo[a]pyrene

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