Carcinogenesis, Teratogenesis & Mutagenesis ›› 2010, Vol. 22 ›› Issue (6): 444-447.doi: 10.3969/j.issn.1004-616x.2010.06.008

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Detecting EGFR mutations in non_small cell lung carcinoma patients using high resolution melting curve analysis

YOU Tian1，2, LIU Zheng2, QIU Guang-bin2，   

  1. 1. Department of Emergency Medicine, the First Clinical School of China Medical University, Shenyang 110001; 2.Department of Laboratory Medicine,No.202 Hospital of PLA, Shenyang 110003,Liaoning, China
  • Received:2010-07-15 Revised:2010-09-25 Online:2010-11-30 Published:2010-11-30
  • Contact: QIU Guang-bin

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Abstract: OBJECTIVE: To evaluate the feasibility and sensitivity of detecting the epidermal growth factor receptor(EGFR) mutation in non_small cell lung carcinoma(NSCLC) patients using high resolution melting(HRM) curve analysis. METHODS: HRM was applied to detect the mutations of EGFR exon 19 and 21 in NSCLC patients, the results were then confirmed by direct sequencing. RESULTS: Three melting curves were obtained by HRM anlysis for EGFR exon 19 and two melting curves for exon 21. Type A(45/52), B(5/52), C(2/52) melting curves represent wild type , heterozygous delE746_A750 and delL747_S752 of exon 19, respectively.Type N(49/52) and M(3/52) melting curves represent wild type and heterozygous L858R of exon 21. Results of HRM were consistent with those of direct sequenc. CONCLUSION: HRM is an accurate technique to detect EGFR mutations. It is also a low_cost test which is suitable for screening.

Key words: non_small cell lung carcinoma, epidermal growth factor receptor, high resolution melting curve