Carcinogenesis, Teratogenesis & Mutagenesis ›› 2010, Vol. 22 ›› Issue (6): 418-422.doi: 10.3969/j.issn.1004-616x.2010.06.002

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A pilot study of differentially expressed microRNAs between esophageal squamous cell carcinoma and adjacent tissues in Huai'an population

LIU Hui1, LIU Ran1, SU Yao-yao1,YANG Miao1,PU Yue-pu1,YIN Li-hong1,,WANG Yi2,PAN En-chun2,GUO Wei3,YOU Zhen-bin3   

  1. 1. Key Laboratory of Environmental Medical Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009; 2. Huai'an Center for Disease Control and Prevention, Huai'an 223001; 3. Huai'an First People's Hospital, Huai'an 223000,Jiangsu, China
  • Received:2010-06-08 Revised:2010-10-13 Online:2010-11-30 Published:2010-11-30
  • Contact: YIN Li-hong

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Abstract: OBJECTIVE: To study the difference of microRNA expression profiles between esophageal squamous cell carcinoma(ESCC) tissues and adjacent tissues in Huai'an．The result of this study may provide theoretical support and experimental basis for further research on mechanism of microRNAs in the pathogenesis of esophageal squamous cell carcinoma. METHODS: Cancer tissues and corresponding normal esophageal tissues(NET) adjacent to tumor from 3 patients with newly diagnosed ESCC were obtained. Endogenous microRNAs were determined by high_throughput microRNA microarray．RESULTS: Among the 866 human relevant microRNA probes detected by microRNA microarray, the difference of fifteen microRNAs between esophageal cancer tissues and tissues adjacent to the tumors was statistically significant(P<0.05), including a set of 7 overexpressed microRNAs(hsa_miR_20a, hsa_miR_155, hsa_miR_574_5p, hsa_miR_21, hsa_miR_183, hsa_miR_601, hsa_miR_623) and 8 under_expressed microRNAs(hsa_miR_186, hsa_miR_10a, hsa_miR_144, hsa_miR_338_3p, hsa_miR_192, hsa_miR_218, hsa_miR_451, hsa_miR_139_5p). CONCLUSION: There was a special profile of differentially expressed microRNAs between ESCC and NET, which may be associated with the occurrence and development of esophageal squamous cell carcinoma.

Key words: esophageal squamous cell carcinoma, microRNA, differential expression, microarray