氯化镉致人肝癌细胞SMMC-7721线粒体损伤作用

doi:10.3969/j.issn.1004-616x.2010.04.006

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2010, Vol. 22 ›› Issue (4): 276-278.doi: 10.3969/j.issn.1004-616x.2010.04.006

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Mitochondrial damage in SMMC-7721 cells induced by cadmium chloride

LIU Xiao-mei1;SHI Lei1;WANG Wen1;JIN Ming-hua1;DU Hai-ying1;LIU Ying1;SUN Lei1;SUN Zhi-wei1;2

1. School of Public Health, Jilin University, Changchun 130021； 2. School of Public Health and Family Medicine, Capital Medical University, Beijing 100069,China

Received:2009-10-20 Revised:2010-01-30 Online:2010-07-30 Published:2010-07-30
Contact: SUN Zhi-wei

Abstract

Abstract: OBJECTIVE: The mitochondrial damage of SMMC-7721 cells induced by cadmium chloride was studied in vitro. METHODS: The SMMC-7721 cells were treated with cadmium chloride for 24 hours. Morphology was used to assess integrity of mitochondria. Spectrophotography was used to measure the transport ability of Ca2＋ into mitochondria and agarose gel electrophoresis was used to observe the mitochondrial DNA damage. RESULTS: The mumber of treated cell and mitochondria decreased with increasing cadmium concentration. The transport ability of Ca2＋ into mitochondria was decreased and the mitochondrial DNA was damaged with increasing concentration. CONCLUSION: Cadmium chloride could damage the mitochondria and its DNA in SMMC-7721 cells.

Key words: cadmium chloride, SMMC-7721, mitochondria, calciumion

CLC Number:

R994.6

LIU Xiao-mei, SHI Lei, WANG Wen, JIN Ming-hua, DU Hai-ying, LIU Ying, SUN Lei, SUN Zhi-wei, . Mitochondrial damage in SMMC-7721 cells induced by cadmium chloride[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2010, 22(4): 276-278.

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Mitochondrial damage in SMMC-7721 cells induced by cadmium chloride

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