Abstract: OBJECTIVE: To examine the expression of relative genes, screened by gene chip, in transformation of human bronchial epithelial cells induced by glycidyl methacrylate. METHODS: Real time polymerase chain reaction was used to measure the expression of C19orf20, RGS14, EPB49, PSCA, CENPF, CTGF and RASD1, involving in mitosis and signal transduction, in transformation of human bronchial epithelial cells induced by glycidyl methacrylate. RESULTS: Expressions of C19orf20 and RGS14 were up-regulated by 421％ and 178％, respectively, in the transformed 10th- generation(protophase) cells. CENPF and CTGF were down-regulated by 93％ and 77％, respectively, in the transformed 30th-generation(anaphase) cells. EPB49 and PSCA were up-regulated by 191％ and 593％, respectively, in the malignant transformation protophase cells, and by 116％ and 119％, respectively, in the malignant transformation anaphase cells. RASD1 expression was 231％ up-regulated in the malignant transformation protophase cells while 74％ down-regulated in the malignant transformation anaphase cells. CONCLUSION: The malignant transformation of human bronchial epithelial cells is a complicated process involving many genes. The alteration of the above genes may be important molecular events during the transformation of human bronchial epithelial cells induced by glycidyl methacrylate.

Key words: glycidyl methacrylate, human bronchial epithelial cells, malignant transformation, gene expression