Carcinogenesis, Teratogenesis & Mutagenesis ›› 2010, Vol. 22 ›› Issue (3): 171-174.doi: 10.3969/j.issn.1004-616x.2010.03.003

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Utilization of insulin promoter in differentiation of liver progenitor cells

JIN Cai-xia1;2;LI Wen-lin1;LI Yuan-jie2;XU Fang2;HU Yi-ping1   

  1. 1.Department of Cell Biology, College of Basic Medical Science, Second Military Medical University, Shanghai 200433; 2.Department of Medical Genetics and Cell Biology, Ningxia Medical University, Yinchuan 750004
  • Received:2009-10-09 Revised:2010-01-05 Online:2010-05-30 Published:2010-05-30
  • Contact: HU Yi-ping;XU Fang

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Abstract: OBJECTIVE: To construct the report gene expression vector for studying the transdifferentiation potency of liver epithelial progenitor cells (LEPCs). METHODS: The rat insulin Ⅰ promoter(RIP) was amplified by PCR from rat tail genomic DNA and subcloned into promoter-removed pDsRed1-1 vector. By using the reporter gene tagging cells, the phenotype variation of LEPCs transdifferentiate into pancreas-like cells could be evaluated. RESULTS: RIP report gene expression vector could monitor the transdifferentiation of LEPCs into pancreas-like cells in a real-time manner. CONCLUSION: The reporter vector could useful for studying the transdifferentiation of LEPCs into pancreas-like cells.

Key words: liver epithelial progenitor cells, rat insulin promoter, transdifferentiation, red fluorescent protein

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