Carcinogenesis, Teratogenesis & Mutagenesis ›› 2010, Vol. 22 ›› Issue (2): 141-145.doi: 10.3969/j.issn.1004-616x.2010.02.016

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Isolation and culture of ovarian cancer-initiating spheroid cells from primary human tumors

WANG Qi1;TIAN Dong-ping1;LI Cong-zhu2;ZHONG Wei-xiang1;DING Wen-shuang1   

  1. 1. Department of Pathology, Shantou University Medical College, Shantou 515041; 2.Affiliated Tumor Hospital of Shantou University Medical College, Shantou 515031, Guangdong, China
  • Received:2009-10-27 Revised:2009-11-26 Online:2010-03-30 Published:2010-03-30
  • Contact: TIAN Dong-ping

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Abstract: OBJECTIVE: To improve the culture methods of ovarian cancer-initiating spheroid cells. METHODS: The tumor spheroid cells were isolated by tissue culture and collagenase I digestion, then high concentration of serum and serum-free medium were used. Percoll solution was used to isolate tumor spheroid cells after 3 or 5 days. and it was then cultured in suspension about one month. Tumor spheroid cells were identified by light microscope and immunocytochemical method stained for CD44, CD117 and CD34. RESULTS: Both culture methods resulted in growth of tumor spheroid cells. Collagenase digestion method produced more tumor spheroid cells, and CD44 may be a specific antigen marker for the human ovarian cancer. CONCLUSION: Using medium with high concentration of serum could yield a certain amount of tumor spheroid cells. Ovarian cancer-initiating spheroid cells may express CD44 antigen.

Key words: human ovarian cancer-initiating spheroid cells, culture, isolation

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